DR. HARRY MIGUEL GREEN, O.D.
Optometry at Minor Hall, Berkeley, CA
License number
California 13578T
Category
Optometry
Type
Optometrist
Address
Address
200 Minor Hall, Berkeley, CA 94720
Phone
(510) 642-0749
Professional information
Harry Miguel Green, Berkeley CA
Specialties:
Optometrist
Address:
200 Minor Hall, Berkeley, CA 94720
Harry M Green, Berkeley CA - OD (Doctor of Optometry)
Specialties:
Optometry
Address:
200 Minor Hall, Berkeley 94720
(510) 643-2020 (Phone)
(510) 643-2020 (Phone)
Languages:
English
Detection Of Protein Expression In Vivo Using Fluorescent Puromycin Conjugates
US Patent:
2006005, Mar 16, 2006
Filed:
Jun 7, 2005
Appl. No.:
11/147813
Inventors:
Harry Green - Berkeley CA, US
Richard Roberts - South Pasadena CA, US
Erin Schuman - Pasadena CA, US
William Smith - La Jolla CA, US
Bruce Hay - Los Angeles CA, US
Richard Roberts - South Pasadena CA, US
Erin Schuman - Pasadena CA, US
William Smith - La Jolla CA, US
Bruce Hay - Los Angeles CA, US
Assignee:
CALIFORNIA INSTITUTE OF TECHNOLOGY - Pasadena CA
International Classification:
A61K 49/00, C07K 14/47
US Classification:
424009600, 530352000
Abstract:
Disclosed is a class of reagents for examining protein expression in vivo that does not require transfection, radiolabeling, or the prior choice of a candidate gene. Further, a series of puromycin conjugates was constructed bearing various labeling moieties. These conjugates were readily incorporated into expressed protein products in cell lysates in vitro and efficiently cross cell membranes to function in protein synthesis in vivo as indicated by flow cytometry, selective enrichment studies, and western analysis. The present invention demonstrates that labeled-puromycin conjugates offer a general means to examine protein expression in vivo.