Harold Philip Swerdlow
Land Surveyors in Salt Lake City, UT
License number
Utah 176632-9925
Issued Date
Jan 1, 1911
Expiration Date
Dec 31, 1999
Category
Engineer/Land Surveyor
Type
Engineer in Training - Obsolete
Address
Address
Salt Lake City, UT
Professional information
On-Line Dna Analysis System With Rapid Thermal Cycling
US Patent:
5935522, Aug 10, 1999
Filed:
Jun 30, 1997
Appl. No.:
8/885625
Inventors:
Harold P. Swerdlow - Salt Lake City UT
Carl T. Wittwer - Salt Lake City UT
Carl T. Wittwer - Salt Lake City UT
Assignee:
University of Utah Research Foundation - Salt Lake City UT
International Classification:
G01N 3510
US Classification:
422 70
Abstract:
An apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column.