Inventors:
Mikeljon Nikolich - Takoma Park MD, US
David Hoover - Rockville MD, US
R. Roop - Greenville NC, US
Gregory Robertson - Dallas TX, US
International Classification:
A61K039/10, C12N015/74, C12N001/21
US Classification:
424252100, 435252300, 435471000
Abstract:
Live attenuated vaccines against brucellosis are described. New mutant strains of have been developed, which are attenuated via deletion of the hfq and/or purEK sites. The purEK deletion site does not include insertion of a kanamycin resistance determinant marker or any other introduced antibiotic resistance marker. The hfq deletion site preferably does not include insertion of a kanamycin resistance determinant marker or any other introduced antibiotic resistance marker.