Work:
University College London
Ph.D. candidate
King's College London - London
Research Assistant
The National Center of Microscopy and Imaging Research/UCSD - La Jolla, CA
Undergraduate Technician/Staff Research Associate
The Burhnam Institute - La Jolla, CA
Laboratory Assistant
UCSD English Language Program
Conversation Leader
Education:
University College London - London
Ph.D. in Physiology/Epithelial transport
University of California San Diego - La Jolla, CA
B.Sc. in Biochemistry/Cell Biology, Minor: English Literature
Lynbrook High School - San Jose, CA
Skills:
In-vivo surgery/physiology cannulations in anesthetized Sprague Dawley rats (jugular vein, femoral artery, trachea, bladder, intestine) Phosphate balance studies (32P instillation into gut, serum collection, scintillation counter) Glomerular filtration rate determination (FITC-inulin infusions and assays) Mean arterial blood pressure monitoring (Adinstruments Powerlab 30 and software) Ex-vivo gut perfusions Brush border membrane vesicle preparation (kidney and gut) Tissue Culture Culturing and plating immortalized cell lines (HeLa, MDCK, NRK, HEK293, Phoenix, CHL1610), primary cultures (fibroblasts) and EBV transformed lymphoblasts Transfection (pcDNA3.1 and other CMV-based expression vectors) and transduction (MoMuLV, pCLNCX expression vector). Transductions were performed using recombinant viruses generated either in Phoenix cells or HEK293T cells. Proliferation assays (Alamar Blue) Analysis / sorting at the flow cytometer. Single cell cloning, bulk cloning (by serial dilution, cloning rings, or sorting) Immunostaining/Imaging Fluorescent Immunocytochemistry (preparation of fixatives, detergent solutions, blocking agents; fluorescent multicolor labeling, up to 4 simultaneous fluorophores; ReAsh labeling for tetracysteine-tagged DNA; biotin/streptavidin and HRP expression amplification; labeling with Quantum Dots). Confocal microscopy and live-imaging of eukaryotic cell samples (skilled with Olympus Fluoview1000/Zeiss LSM 510 microscopes) Image processing (highly skilled in Adobe Photoshop, ImageJ, Bitplane Imaris, Zeiss LSM software) Immunohistochemistry on paraffin embedded blocks (DAB staining) Preparation of samples for electron microscopy (photooxidation of samples using FRET with ReAsh/DAB method, staining and counter staining with osmium tetroxide and uranyl acetate, embedding with Durcupan resin) Molecular/Biochemical/Misc Western Blots (PAGE) (semi dry transferring, probing with HRP secondaries, ECL detection and GraphPad Prism data analysis) Transformation (DH5alpha and XL1Blue), inoculation, small, medium and large scale culture for plasmid DNA isolation (Quiagen kits). Sub-cloning (mcherry vector - PCR, sequencing etc) Surgical isolation of hepatocytes from mice (post mortem) Basic script writing (MatLab software) Graduate Teaching Assistant/Demonstrator (University College London) Home Office Personal License (UK Animal Handling certificate)