GLENN SPAULDING, M.D.
Osteopathic Medicine at Soaring Forest Dr, Houston, TX

5369012, Nov 29, 1994

Mar 26, 1992

7/857901

Steven L. Koontz - Seabrook TX
Glenn F. Spaulding - Houston TX

The United States of America as represented by the Administrator of the
National Aeronautics and Space Administration - Washington DC

G01N 33545, G01N 33535, C12N 1108, C12N 500

435 792

A portion of an organic polymer article such as a membrane is made hydrophilic by exposing a hydrophobic surface of the article to a depth of about 50 to about 5000 angstroms to atomic oxygen or hydroxyl radicals at a temperature below 100. degree. C. , preferably below 40. degree. C. , to form a hydrophilic uniform surface layer of hydrophilic hydroxyl groups. The atomic oxygen and hydroxyl radicals are generated by a flowing afterglow microwave discharge, and the surface is outside of a plasma produced by the discharge. A membrane having both hydrophilic and hydrophobic surfaces can be used in an immunoassay by adhering antibodies to the hydrophobic surface. In another embodiment, the membrane is used in cell culturing where cells adhere to the hydrophilic surface. Prior to adhering cells, the hydrophilic surface may be grafted with a compatibilizing compound. A plurality of hydrophilic regions bounded by adjacent hydrophobic regions can be produced such that a maximum of one cell per each hydrophilic region adheres.

5496722, Mar 5, 1996

May 25, 1993

8/066292

Thomas J. Goodwin - Friendswood TX
David A. Wolf - Houston TX
Glenn F. Spaulding - Houston TX
Tacey L. Prewett - Friendswood TX

The United States of America as represented by the Administrator of the
National Aeronautics and Space Administration - Washington DC

C12N 500, C12N 502, C12N 506, C12N 508, C12M 302, C12M 110, A01N 102

43524023

Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under microgravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.

5858783, Jan 12, 1999

Mar 1, 1996

8/613793

Thomas J. Goodwin - Friendswood TX
David A. Wolf - Houston TX
Glenn F. Spaulding - Houston TX
Tacey L. Prewett - Friendswood TX

The United States of America as represented by the Administrator of the
National Aeronautics and Space Administration - Washington DC

C12N 500, C12N 502

435373

Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under micro-gravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated micro-gravity created in a horizontal rotating wall culture vessel. The medium used for culturing the cells, especially a mixture of epithelial and mesenchymal cells contains a mixture of Mem-alpha and Leibovits L15 supplemented with glucose, galactose and fructose.

2011001, Jan 20, 2011

Jul 13, 2010

12/835386

Glenn F. Spaulding - Houston TX, US

MICROSCOPES INTERNATIONAL, LLC - Plano TX

A61B 5/00, H04N 7/18, H04N 5/225, G06K 9/00, G01J 3/46, G06Q 50/00, G06Q 10/00, G06Q 40/00

600300, 348 79, 3482071, 382128, 356402, 705 2, 348E07085, 348E05024

A home healthcare management system wherein a patient conducts self diagnoses and self testing, and manages their own medical records at home. A digital microscope is utilized as part of the system that is smaller, of lower cost, faster, of a higher dynamic range, and has a higher resolution than conventional microscopes. The microscope consists of an illumination source, a spatial sub-sampling device and a detector device. The digital microscope provides a vectored method of collecting images from a digital microscope that is independent of the optical resolution, and a slide based coordinate system, and a method of displaying images and communicating such images over the Internet in a file format that does not require a header or prior knowledge of magnification, coordinate system, or tiling structure. The system further includes an interface for physiological monitoring devices and a connection to the Internet for more comprehensive services.

7355698, Apr 8, 2008

Apr 24, 2006

11/379845

Shishir Shah - Houston TX, US
Glenn F. Spaulding - Houston TX, US

Spin Diagnostics, Inc. - Houston TX

G01N 21/03

356246

An imaging device and system for the high throughput imaging of multi-sample containers is described. Generally speaking, one or more containers capable of holding multiple sample volumes are fixed to a platform which is rotated during image acquisition operations. Images of sample volumes are obtained by moving an imaging device (e. g. , a camera) from a first position to a second position across the one or more sample volumes. Because the platform is continuously rotated there is no lost time due to moving the samples (e. g. , acceleration, deceleration and backlash compensation). Movement artifacts associated with motion of the imaging device occur in those times when the imaging device is “between” sample volumes, thereby eliminating any lost time due to this motion. Rather than, or in combination with, the aforementioned lights sources, additional light sources may be added to provide epi-illumination.

5523228, Jun 4, 1996

Jul 7, 1995

8/499348

Marylou Ingram - Pasadena CA
Glenn F. Spaulding - Houston TX
James J. G. Craft - Pasadena CA
Chuen P. Ng - Los Angeles CA
Ramez Saroufeem - Pasadena CA
Geza B. Techy - Monrovia CA
Ozkan Yazan - Norwalk CA

HMRI/CLMF - Houston TX

C12N 502

43524025

A cell culture chamber with a novel hydrodynamic environment, constructed with gas permeable membranes is disclosed. The culture chamber is horizontally rotated, wall movement suspends cells and enhances oxygen mass transfer as cells aggregate and grow into 3-dimensional tissue.

5330908, Jul 19, 1994

Dec 23, 1992

7/996263

Glenn F. Spaulding - Houston TX

The United States of America as represented by the Administrator,
National Aeronautics and Space Administration - Washington DC

C12N 502, C12M 124, C12M 104, C12M 110

43524024

An annular culture vessel for growing mammalian cells is constructed in a one piece integral and annular configuration with an open end which is closed by an endcap. The culture vessel is rotatable about a horizontal axis by use of conventional roller systems commonly used in culture laboratories. The end wall of the endcap has tapered access ports to frictionally and sealingly receive the ends of hypodermic syringes. The syringes permit the introduction of fresh nutrient and withdrawal of spent nutrients. The walls are made of conventional polymeric cell culture material and are subjected to neutron bombardment to form minute gas permeable perforations in the walls.

2003008, May 8, 2003

Jul 25, 2002

10/205431

Glenn Spaulding - Houston TX, US
Anthony Spaulding - Houston TX, US

F41J005/02, G01N021/86

273/317000, 273/348000, 250/222200, 250/221000, 250/559320

A novel simple low cost paintball velocimeter is described. It is small, utilizing a light source and photodetection methods that can serve as a stand-alone velocimeter or integrated into the firing mechanism for greater control and accuracy. Additional features include an ability to collect, store and process data for graphical display and analysis.

6117674, Sep 12, 2000

Dec 29, 1994

8/366065

Thomas J. Goodwin - Houston TX
Glenn F. Spaulding - Houston TX
David A. Wolf - Houston TX

The United States of America as represented by the Administrator of the
National Aeronautics and Space Administration - Washington DC

C12N 500

435325

A process for propagating a pathogen in a three-dimensional tissue mass cultured at microgravity conditions in a culture vessel containing culture media and a culture matrix is provided. The three-dimensional tissue mass is inoculated with a pathogen and pathogen replication in the cells of the tissue mass achieved.

6001643, Dec 14, 1999

May 22, 1998

9/083515

Glenn F. Spaulding - Houston TX

C-Med Inc. - Houston TX

C12M 302

4352982

A novel hydrodynamic cell culture environment is disclosed for a two-chamber roller bottle. The unstable hydrodynamics of a gas headspace media chamber is coupled through a center opening to a second zero headspace culture chamber. Coupling the two chambers creates novel fluid streamlines that add turbulence for improved mass transfer and slow media repletion. The exchange between chambers regulates turbulence and thus concomitant exchange of nutrients i. e. , environmental control. It is disposable, automatable, and suited to culturing tissue for transplants.