Inventors:
Malford Cullum - Grayslake IL, US
Gerald Mazurek - Dunwoody GA, US
International Classification:
C12Q001/70, G01N033/53, G01N033/569, G01N033/554
US Classification:
435005000, 435007220, 435007320
Abstract:
The inventive subject matter relates to a competitive method for the diagnosis of latent infectious disease, such as Mycobacterium tuberculosis, by estimating, the concentration of cytokine, such as interferon-gamma produced by stimulated immune cells, collected from whole blood, by Fluorescence Polarization (FP), Fluorescence Resonance Energy Transfer (FRET) or Fluorescence Lifetime (FLT) due to antibody-cytokine interaction or by dimerization of the cytokine.