Inventors:
Francis P. McCormick - Berkeley CA
Gail L. Wong - Oakland CA
Robin Clark - San Leandro CA
Norman Arnheim - Piedmont CA
Danute E. Nitecki - Berkeley CA
James R. Feramisco - Cold Spring Harbor NY
Assignee:
Cetus Corporation - Emeryville CA
Cold Spring Harbor Laboratories - Cold Spring Harbor NY
International Classification:
A61K 39395
Abstract:
Antibodies exhibit specificity toward single amino acid differences between proteins. These antibodies may be produced by synthesizing a peptide of the appropriate amino acid sequence contained in the protein, immunizing a host with the peptide, and extracting sera from the host to obtain the antibodies. The antibodies and the desired protein are then immunoprecipitated under conditions of partial denaturation to expose the epitope of the protein. The antibodies may be used for diagnostic or therapeutic purposes.