FREDERICK V PLAPP, MD PHD
Medical Practice at Wornall Rd, Kansas City, MO

4816413, Mar 28, 1989

Sep 8, 1986

6/904948

Lyle T. Sinor - Merriam KS
Frederick V. Plapp - Overland Park KS
Jane M. Rachel - Kansas City MO

Immucor, Inc. - Norcross GA

G01N 33555, G01N 33549

436520

A method and composition for use in preparing red blood cells for use as indicator cells to detect the presence of antigens and antibodies in biological fluids is the subject of the present invention. Freshly collected human or animal erythrocytes are washed six times with isotonic saline, and suspended to a hematocrit of four percent (volume/volume) with isotonic saline. An incomplete antibody, specific for the antigen or antibody to be detected, in a quantity less than that which would cause irreversible agglutination, is added to the erythrocyte solution. The mixture is incubated, washed, and suspended to a hematocrit of 3-5 (v/v) with isotonic saline. A second antibody, immuno specific for the first antibody and in an amount sufficiently great to immunologically bind substantially all of the antibody carrying red blood cells but not so great as to cause irreversible agglutination, is attached to the erythrocytes. This mixture is incubated, washed, and suspended to a hematocrit of 0. 1-1% (v/v) in a media containing red blood cell nutrients.

4841818, Jun 27, 1989

Feb 1, 1988

7/150541

Christopher V. Plapp - Overland Park KS
Frederick A. Plapp - Mission KS
Frederick V. Plapp - Overland Park KS

B25B 2700

81 308

A device and method for removing closures from laboratory sample containers is the subject of the present invention. The device consists of a tubular member which is large enough to contain a laboratory sample container or vial. Integral with one end of the tubular member is a housing member which is large enough to contain the stopper or closure for the laboratory vial and is thus of a diameter larger than the tubular member. The end of the housing opposite the tubular member is closable with an irreversibly locking cap. A vial with a stopper to be removed is placed into the cap end of the housing member and is received by the tubular member unitl the stopper rests on the bottom surface of the housing member. The cap on the housing member is closed and a force is exerted on the vial to dislodge the stopper from it. The vial is pulled through the tubular member, leaving the stopper in the housing member.