DR. FREDERIC M WALDMAN, MD
Medical Practice at Divisadero St, San Francisco, CA

7094534, Aug 22, 2006

Jul 24, 2001

09/912818

Daniel Pinkel - Walnut Creek CA, US
Joe W. Gray - San Francisco CA, US
Anne Kallioniemi - Rockville MD, US
Frederic Waldman - San Francisco CA, US
Masaru Sakamoto - Tokyo, JP

The Regents of the University of California - Oakland CA

C12Q 1/68, C12P 19/34, C07H 21/02

435 6, 435 912, 536 231

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

8021837, Sep 20, 2011

Feb 24, 2006

11/361316

Daniel Pinkel - Walnut Creek CA, US
Joe W Gray - San Francisco CA, US
Anne Kallioniemi - Tampere, FI
Ollie-Pekka Kallioniemi - Turku, FI
Frederic Waldman - San Francisco CA, US
Masaru Sakamoto - Chiyoda-ku, JP

The Regents of the University of California - Oakland CA

C12Q 1/68, G01N 33/566, G01N 33/53, C07H 21/02, C07H 21/04

435 6, 436501, 436813, 536 231, 536 243

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

6335167, Jan 1, 2002

May 14, 1999

09/311835

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Rockville MD
Frederic Waldman - San Francisco CA
Masaru Sakamoto - Tokyo, JP

The Regents of the University of California - Oakland CA

C12Q 168

435 6, 435 712, 536 231, 536 243

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

6159685, Dec 12, 2000

Jul 30, 1997

8/903095

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Tampere, FI
Olli-Pekka Kallioniemi - Tampere, FI
Frederic Waldman - San Francisco CA

The Regents of the University of California - Oakland CA

C12Q 168, C12P 1934, C07H 2102, C07H 2104

435 6

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

5965362, Oct 12, 1999

Nov 27, 1995

8/562965

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Tampere, FI
Olli-Pekka Kallioniemi - Tampere, FI
Frederic Waldman - San Francisco CA
Masaru Sakamoto - Tokyo, JP

The Regents of the University of California - Oakland CA

C12Q 168, C12P 1934, C07H 2102, A61K 4900

435 6

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

5665549, Sep 9, 1997

Jun 6, 1995

8/466122

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Tampere, FI
Olli-Pekka Kallioniemi - Tampere, FI
Frederic Waldman - San Francisco CA

The Regents of the University of California - Oakland CA

C12Q 168, C12P 1934, C07H 2102, C07H 2104

435 6

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

7537895, May 26, 2009

May 8, 2006

11/431094

Daniel J. Pinkel - Walnut Creek CA, US
Joe W Gray - San Francisco CA, US
Anne Kallioniemi - Tampere, FI
Olli-Pekka Kallioniemi - Tampere, FI
Frederic Waldman - San Francisco CA, US

The Regents of the University of California - Oakland CA

C12Q 1/68, C12Q 1/70, C12P 19/34

435 6, 435 5, 435 912, 435 911, 536 231, 536 2432, 536 2433

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

5856097, Jan 5, 1999

Nov 27, 1995

8/562898

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Tampere, FI
Olli-Pekka Kallioniemi - Tampere, FI
Frederic Waldman - San Francisco CA
Masaru Sakamoto - Tokyo, JP

The Regents of the University of California - Oakland CA

C12Q 168, C12Q 170, C12P 1934, C07H 2104

435 6

Disclosed are new methods comprising the use, of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

5721098, Feb 24, 1998

Jun 6, 1995

8/468629

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Tampere, FI
Olli-Pekka Kallioniemi - Tampere, FI
Frederic Waldman - San Francisco CA

The Regents of the University of California - Alameda CA

C12Q 168, C12P 1134, C07H 2102, C07H 2104

435 6

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).