DR. ERIC O. FEIGL, MD
Osteopathic Medicine in Seattle, WA

5931779, Aug 3, 1999

Jun 6, 1997

8/870483

Lorilee S. Arakaki - Narberth PA
Eric Feigl - Seattle WA
Martin J. Kushmerick - Seattle WA
David Marble - Seattle WA
David H. Burns - Montreal, CA
Kenneth H. Schenkman - Madison WI

Wisconsin Alumni Research Foundation - Madison WI

A61B 500

600310

A method for determining myoglobin oxygen fractional saturation in vivo in muscle tissue and intracellular oxygen tension (pO. sub. 2), typically in the presence of hemoglobin, is provided. The method comprises measuring the absorption spectrum of the tissue using spectrographic equipment known to the art, including equipment for non-invasively taking spectroscopic measurements of tissue, or adaptations of such equipment to provide the preferred measurements described herein, or using spectrographic equipment specifically designed as described herein for use in non-invasive measurements of reflectance spectra. The measured spectrum is corrected for light scattering effects, such as by taking the second derivative of the data, or by using other means which are known to the art. Myoglobin fractional oxygen saturation is calculated from the measured spectrum employing calibration coefficients that are themselves calculated from application of multivariate analysis to a calibration set created from the second derivatives of absorption spectra. The calibration set spectra preferably representing absorbances of (1) a range of concentrations of hemoglobin (wherein a range of concentrations of oxyhemoglobin and deoxyhemoglobin are present), (2) one concentration of myoglobin (preferably selected to match the concentration of myoglobin in the target muscle tissue) with varying relative amounts of oxy- and deoxymyoglobin and (3) a range of concentrations of scattering agents to mimic scattering encountered in target tissue.

2008020, Aug 21, 2008

Nov 29, 2007

11/947242

Kenneth A. Schenkman - Seattle WA, US
Lorilee S.L. Arakaki - Bainbridge Island WA, US
Wayne A. Ciesielski - Seattle WA, US
Eric O. Feigl - Seattle WA, US
David H. Burns - Point Claire, CA

A61B 5/00

600310

This document discloses, among other things, systems and methods for measuring a level of myoglobin, hemoglobin, and cytochrome using an optical probe coupled to a spectrometer. Multivariate analysis of the spectral data yields quantifiable cellular and mitochondrial characteristics.