Inventors:
GONGXIN YU - BOISE ID, US
ERIC BROWN - BOISE ID, US
HENRY A. CHARLIER - BOISE ID, US
Assignee:
BOISE STATE UNIVERSITY - BOISE ID
International Classification:
G01N 33/00
Abstract:
The proteins in a cell are preferably proteolytically cleaved and chemically attached to another peptide of unique and known sequence. In one embodiment of the invention, peptide-linker-peptide triplets are synthesized with linker molecules such as polyhistidine. In a more preferred embodiment of the invention, peptide-mass differentiated group (MDG) constructs are synthesized. The MDG's may be obtained from a library of oligo-N(K)-peptides synthesized on resin beads, wherein N is the length of the peptides (with a default value of 4) and K is the number of alternative amino acids (with a default value of 10) at each position. Coupling between given peptides and linkers or MDG's creates recombinants with different overall masses that migrate separately in chromatographic separations. The peptides-linker/MGD's recombinants may be purified and sequenced by MS/MS analysis. The resulting purified and sequenced peptides are then counted, and the ratios of the different peptides within and/or between samples obtained.