Work:
Burkhardt Laboratory, Children's Hospital of Philadelphia - Philadelphia, PA
Graduate Student
Koretzky Laboratory, University of Pennsylvania - Philadelphia, PA
Research Specialist
Education:
University of Pennsylvania, School of Medicine - Philadelphia, PA
Ph.D. in Immunology
Haverford College - Haverford, PA
B.S. in Biology
Skills:
Assays: DNA isolation, DNA primer design, PCR, gel electrophoresis, molecular cloning, transfection, viral transduction, gene expression, SDS-PAGE, western analysis, immunoprecipitation, cell culture, multicolor flow cytometry, FACS, microscopy, ELISA, immunofluorescence, multiphoton intravital microscopy, transwell migration, and collagen 3D migration. Animals: Mouse handling, mouse surgery, retro-orbital injections, intravenous tail injections, footpad injections, genotyping, breeding, and organ harvesting (bones, lymph nodes, spleen, and thymus). Analysis: FlowJo, ImageJ, Slidebook, Volocity, MATLAB, KaleidaGraph, Adobe Illustrator, Adobe Photoshop, EndNote, Microsoft Excel, Microsoft Powerpoint, and Microsoft Word. Cell lines: 293T, 3B-11, 3T3, and Jurkat.