Inventors:
Gerardo Castillo - Seattle WA, US
Paula Y. Choi - Bothell WA, US
Elizabeth Nguyen - Renton WA, US
Alan D. Snow - Lynnwood WA, US
Assignee:
ProteoTech, Inc. - Kirkland WA
International Classification:
A51K035/78, A61K031/35
Abstract:
Assay-guided affinity fractionation and reverse phase high pressure liquid chromatography (HPLC) methodology to isolate, test and characterize the most active water-soluble ingredients within Cat's Claw, or. These components appear to account for the majority of the amyloid or Aβ fibrillogenesis inhibitory activity. Individual fractions and/or compounds as isolated by HPLC are tested in relevant in vitro and/or animal models, and found to consistently demonstrate inhibition of amyloid or Aβ fibrillogenesis. Related extraction methods are disclosed.