Inventors:
Elias T. Zambidis - Baltimore MD, US
Tea Soon Park - Baltimore MD, US
Assignee:
THE JOHNS HOPKINS UNIVERSITY - Baltimore MD
International Classification:
C12N 5/10, A61K 35/12
US Classification:
424 937, 435373, 435325, 435366
Abstract:
The present invention relates generally to the field of stem cells and, more particularly, to reprogramming blood cells to pluripotent stem cells. In a specific embodiment, a method for producing an induced pluripotent stem cell from a human myeloid progenitor cell comprising the steps of (a) activating the human myeloid progenitor cell by incubation with hematopoietic growth factors; (b) transfecting the activated progenitor cells with a non-viral vector expressing one or more pluripotency factors; and (c) co-culturing the transfected cells with irradiated mesenchymal bone marrow stromal cells.