EDWARD Y. SKOLNIK, M.D.
Osteopathic Medicine at 1 Ave, New York, NY

6391584, May 21, 2002

Mar 29, 1999

09/280598

Joseph Schlessinger - New York NY
Edward Y. Skolnik - New York NY
Benjamin L. Margolis - Ann Arbor MI

New York University Medical Center - New York NY

C07K 14705

435 691, 4352523, 4353201, 530350, 53038822, 536 235

The present invention relates to a novel method, based on direct expression cloning, for identifying target proteins capable of binding to and/or serving as substrates for receptor or cytoplasmic tyrosine kinases. The present invention also relates to novel proteins identified using this method, and to methods for identifying compounds that disrupt the interaction of such novel proteins with the receptor or cytoplasmic tyrosine kinases.

5677421, Oct 14, 1997

Mar 11, 1994

8/208887

Joseph Schlessinger - New York NY
Edward Y. Skolnik - New York NY
Benjamin L. Margolis - New York NY

New York University - New York NY

C12N 1512, C07K 1447

530350

A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probe, a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins/discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.

5858686, Jan 12, 1999

Oct 4, 1995

8/539005

Joseph Schlessinger - New York NY
Edward Y. Skolnik - New York NY
Benjamin L. Margolis - New York NY

New York University - New York NY

G01N 3353

435 78

A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probe, a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.

5434064, Jul 18, 1995

Jun 30, 1992

7/906349

Joseph Schlessinger - New York NY
Edward Y. Skolnik - New York NY
Benjamin L. Margolis - New York NY

New York University - New York NY

C12N 1512

4351723

A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probes a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.

5618691, Apr 8, 1997

Dec 16, 1993

8/167035

Joseph Schlessinger - New York NY
Edward Y. Skolnik - New York NY
Benjamin L. Margolis - New York NY

New York University - New York NY

C12N 510, C12N 1512, C12N 1562

435 691

A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probe, a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.

5889150, Mar 30, 1999

Jun 2, 1994

8/252820

Joseph Schlessinger - New York NY
Edward Y. Skolnik - New York NY
Benjamin L. Margolis - New York NY
Harald App - San Francisco CA

New York University Medical Center - New York NY

C07K 14205, C12N 1512

530350

The present invention relates to a novel method, based on direct expression cloning, for identifying target proteins capable of binding to and/or serving as substrates for receptor or cytoplasmic tyrosine kinases. The present invention also relates to novel proteins identified using this method, and to methods for identifying compounds that disrupt the interaction of such novel proteins with the receptor or cytoplasmic tyrosine kinases.