Inventors:
Christopher Tate - Frederick MD
Russell Miller - Glen Dale MD
Edward Mallinson - Columbia MD
Sammy Joseph - Derwood MD
Assignee:
The University of Maryland - College Park MD
The United States of America as represented by the Secretary of
Agriculture - Washington DC
International Classification:
C12Q 110, C12Q 104, C12Q 124, C12Q 102, C12Q 122, C12Q 106, C12Q 108, C12N 120
Abstract:
An improved plating media incorporates TERGITOL. RTM. 4, an alkyl sodium sulfate biological detergent, in a Xylose-lysine agar base. The plating media may also include peptone and sulfapyridine to further inhibit Citrobacter growth. The media is highly preferential for Salmonella, giving a greater number of Salmonella positives, and reduced competitive organisms. When incorporated with drag swab methodology employing skim milk or evaporated milk as a holding media, which may advantageously include novobiocin to suppress unwanted bacterial growth, coupled with a tetrathionate-culturing broth or other selective enrichment broth, the plating media completes a highly sensitive assay for the detection of Salmonella, particularly keyed for poultry and livestock handling structures, and for veterinary and human clinical laboratory applications.