EDWARD T MALLINSON
Veterinary in Columbia, MD

5208150, May 4, 1993

Apr 8, 1991

7/681358

Christopher Tate - Frederick MD
Russell Miller - Glen Dale MD
Edward Mallinson - Columbia MD
Sammy Joseph - Derwood MD

The University of Maryland - College Park MD
The United States of America as represented by the Secretary of
Agriculture - Washington DC

C12Q 110, C12Q 104, C12Q 124, C12Q 102, C12Q 122, C12Q 106, C12Q 108, C12N 120

435 38

An improved plating media incorporates TERGITOL. RTM. 4, an alkyl sodium sulfate biological detergent, in a Xylose-lysine agar base. The plating media may also include peptone and sulfapyridine to further inhibit Citrobacter growth. The media is highly preferential for Salmonella, giving a greater number of Salmonella positives, and reduced competitive organisms. When incorporated with drag swab methodology employing skim milk or evaporated milk as a holding media, which may advantageously include novobiocin to suppress unwanted bacterial growth, coupled with a tetrathionate-culturing broth or other selective enrichment broth, the plating media completes a highly sensitive assay for the detection of Salmonella, particularly keyed for poultry and livestock handling structures, and for veterinary and human clinical laboratory applications.

5212067, May 18, 1993

Mar 30, 1989

7/330479

Edward T. Mallinson - Columbia MD
Christopher R. Tate - Frederick MD
Russell G. Miller - Glen Dale MD

The United States of America as represented by the Secretary of
Agriculture - Washington DC
The University of Maryland - College Park MD

C12Q 110, C12Q 102, C12Q 124, C12Q 122

435 38

A swift, accurate assay for the detection of salmonella contamination in a livestock environments, comprises sampling the floor of the livestock holding area with drag swabs for about 10 or more minutes, after which said swabs are maintained in a static condition, at reduced temperature, in the presence of double strength skim milk, until ready for testing. The swabs are transferred to a salmonella-preferential growth medium, such as tetrathionate broth and subsequently assayed. To reduce non-salmonella "look alike" or masking bacterial colonies, Novobiocin may be administered to the culture media.

5871944, Feb 16, 1999

Jul 2, 1997

8/887274

Russell G. Miller - Gambrills MD
Edward Mallinson - Columbia MD

The University of Maryland - College Park MD

G01N 33569, C12N 120

435 735

A new Salmonella preferential media uses lactose and cellobiose to suppress formation of black colonies by non-Salmonella, to permit easy inspection of the media to determine the presence or absence of Salmonella. Modified media, including high levels of peptone, beef extract, and tris buffers are required for preferential detection of S. typhi, all other Salmonella and Shigella.