EDWARD N HANLEY, MD
Medical Practice at Morehead Medical Dr, Charlotte, NC

2012023, Sep 20, 2012

Mar 12, 2012

13/418125

Yubo Sun - Charlotte NC, US
Edward N. Hanley - Charlotte NC, US

The Charlotte-Mecklenbury Hospital Authority d/b/a Carolinas Medical Center - Charlotte NC

A61K 31/715, A61K 31/56, A61K 31/28, A61P 19/10, A61K 31/295, A61K 31/225, A61P 19/02, A61P 21/00, A61K 31/66, A61K 31/315

514 54, 514114, 514119, 514121, 514171, 514492, 514494, 514502, 514547

The invention is directed to a composition and associated method for the treatment of musculoskeletal related disorders and diseases. In particular, the present invention provides a class of citrate compounds that can be used to for the treatment of diseases/disorders characterized by the degeneration of musculoskeletal tissues including menisci, bone, articular cartilage, and soft tissues. Examples of suitable citrate and citrate analog compounds include citrates having the following base formula (I):where X may be one of the following:wherein Y, Y′, and Y″ are independently a citrate moiety, O, O—Y, OH, NH, NH—Y, R and R—Y, with R being an alkyl, alkyene, ester, aryl, or phenyl group having from 1 to 6 carbon atoms.

6080579, Jun 27, 2000

Nov 26, 1997

8/979674

Edward Nathaniel Hanley - Charlotte NC
Helen Elizabeth Gruber - Charlotte NC

Charlotte-Mecklenburg Hospital Authority - Charlotte NC

C12N 500, C12N 508

435366

There is provided a method for growing human intervertebral cells. Disc tissue is surgically removed from a normal disc of a patient, the cells expanded by feeding with a cell stimulant such as a growth factor, or a cytokine or a bioactive agent to form monolayer primary cell cultures on a plastic mesh such as a nylon mesh. In the case of a growth factor, fetal bovine serum is preferred as it improves cell proliferation and production of appropriate extracellular matrix components. In another aspect of this invention, the monolayer primary cell cultures are seeded in alginate or agarose and fed again with the cell stimulant until three-dimensional cell cultures are formed. The cells are recovered from the alginate or agarose or from monolayer cultures. Re-implantation is carried out using bioresorbable carriers or cell suspensions.

2005023, Oct 20, 2005

Jun 13, 2005

11/151141

Edward Hanley - Charlotte NC, US
Helen Gruber - Charlotte NC, US

A61K045/00, C12N005/08

424093700, 435368000

There is provided a method for growing human intervertebral cells. Disc tissue is surgically removed from a normal disc of a patient, the cells expanded by feeding with a cell stimulant such as a growth factor, or a cytokine or a bioactive agent to form monolayer primary cell cultures on a plastic mesh such as a nylon mesh. In the case of a growth factor, fetal bovine serum is preferred as it improves cell proliferation and production of appropriate extracellular matrix components. In another aspect of this invention, the monolayer primary cell cultures are seeded in alginate or agarose and fed again with the cell stimulant until three-dimensional cell cultures are formed. The cells are recovered from the alginate or agarose or from monolayer cultures. Re-implantation is carried out using bioresorbable carriers or cell suspensions.