DR. EDWARD JOHN SHELDON, M.D.
Marriage and Family Therapists at Cardiff St, San Diego, CA

6638482, Oct 28, 2003

Dec 2, 1998

09/204324

Donald E. Ackley - Cardiff CA
Edward L. Sheldon - San Diego CA
Michael K. Krihak - San Diego CA

Nanogen, Inc. - San Diego CA

G01N 1500

422 681, 422 50, 422129, 435 6, 435 7, 435 911

Methods and apparatus for use of a stacked, reconfigurable system is provided. The stacked, reconfigurable system includes an inlet for receipt of a sample, a first chamber defined by a bottom support, an intermediate member, and a first spacer, the first chamber being coupled to the inlet through the bottom support, the first chamber including an analysis system having electrodes for electrophoretic transport of material. A second chamber is stacked on top of the first chamber, the second chamber being defined by the intermediate member, a top member, and a second spacer, the second chamber being in fluid communication with the first chamber through at least one via formed in the intermediate member, the second chamber being electrically reconfigurable to permit action such as, for example, synthesis of a compound or specialized analysis. The apparatus can be used to perform analysis on charged biological materials.

6375899, Apr 23, 2002

Dec 2, 1998

09/203730

Donald E. Ackley - Cardiff CA
Edward L. Sheldon - San Diego CA
Michael K. Krihak - San Diego CA

Nanogen, Inc. - San Diego CA

G01N 100

422 681, 422 50, 422 63, 422 69, 422 70, 422 99, 422129, 4352831, 4352851, 4352852, 4352865, 4352873, 4352891, 4352941, 4353051, 4353052

Methods, apparatus, and applications for use of a stacked, reconfigurable system for electrophoretic transport are provided. In one embodiment, a system having a first chamber including at least a bottom support and an intermediate support, and a second chamber, said second chamber including a bottom support and a top member, the first and second chambers being coupled through a via. Electrophoretic, and optional electro-osmotic and thermal, transport is effected. In another aspect of this invention, three or more chambers are coupled by an electrophoretic buss. The electrophoretic buss includes driving electrodes and is adapted to receive fluid containing materials for transport. The chambers are coupled to the electrophoretic buss and serve as a tap from the buss for delivery of charged materials. In one embodiment, certain functions are performed in different chambers. For example, the first chamber may receive the sample and perform sample processing functions, the second chamber may perform amplification procedures, yet a third chamber may perform hybridization or other assays, and yet another chamber may perform immunoassays.

6468744, Oct 22, 2002

Nov 17, 1999

09/341399

Maureen T. Cronin - Los Altos CA
Edward L. Sheldon - San Diego CA
Charles G. Miyada - San Jose CA
Earl A. Hubbell - Los Angeles CA
Mark Chee - Del Mar CA
Stephen P. A. Fodor - Palo Alto CA
Xiaohua C. Huang - Mountain View CA
Robert J. Lipshutz - Palo Alto CA
Peter E. Lobban - Los Altos CA
MacDonald S. Morris - Felton CA

Affymetrix, Inc. - Santa Clara CA

C12Q 168

435 6

The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.

6129828, Oct 10, 2000

Sep 6, 1996

8/709358

Edward L. Sheldon - San Diego CA
Thomas R. Jackson - La Jolla CA
Paul D. Swanson - Santee CA
Bradley S. Scott - San Diego CA
Michael J. Heller - Encinitas CA

Nanogen, Inc. - San Diego CA

B01D 5702, B01D 6142, C12N 1510, G01N 2726

204518

Systems and methods for the electronic sample preparation of biological materials utilize the differential charge-to-mass ratio and/or the differential affinity of sample constituents to separation materials for sample preparation. An integrated system is provided for performing some or all of the processes of: receipt of biological materials, cell selection, sample purification, sample concentration, buffer exchange, complexity reduction and/or diagnosis and analysis. In one embodiment, one or more sample chambers adapted to receive a buffer solution are formed adjacent to a spacer region which may include a trap or other affinity material, electrophoretic motion of the materials to be prepared being effected through operation of electrodes. In another aspect of this invention, a transporter or dipstick serves to collect and permit transport of materials, such as nucleic acids, most preferably DNA and/or RNA. In one embodiment, a membrane or trap is held in a frame which is adapted to mate with a channel formed in the spacer region.

2006022, Oct 12, 2006

Mar 3, 2006

11/367800

Maureen Cronin - Los Altos CA, US
Charles Miyada - San Jose CA, US
Earl Hubbell - Los Angeles CA, US
Mark Chee - Palo Alto CA, US
Stephen Fodor - Palo Alto CA, US
Xiaohua Huang - Mountain View CA, US
Robert Lipshutz - Palo Alto CA, US
Peter Lobban - Mountain View CA, US
MacDonald Morris - Felton CA, US
Edward Sheldon - San Diego CA, US

Affymetrix, INC. - Santa Clara CA

G06F 19/00

702019000, 702020000

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.

6319472, Nov 20, 2001

Dec 2, 1998

9/203935

Donald E. Ackley - Cardiff CA
Edward L. Sheldon - San Diego CA
Michael K. Krihak - San Diego CA

Nanogen, Inc. - San Diego CA

G01N 1506

422 681

Methods, apparatus, and applications for use of a stacked, reconfigurable system for electrophoretic transport are provided. In one embodiment, a system having a first chamber including at least a bottom support and an intermediate support, and a second chamber, said second chamber including a bottom support and a top member, the first and second chambers being coupled through a via. Electrophoretic, and optional electro-osmotic and thermal, transport is effected. In another aspect of this invention, three or more chambers are coupled by an electrophoretic buss. The electrophoretic buss includes driving electrodes and is adapted to receive fluid containing materials for transport. The chambers are coupled to the electrophoretic buss and serve as a tap from the buss for delivery of charged materials. In one embodiment, certain functions are performed in different chambers. For example, the first chamber may receive the sample and perform sample processing functions, the second chamber may perform amplification procedures, yet a third chamber may perform hybridization or other assays, and yet another chamber may perform immunoassays.

6027880, Feb 22, 2000

Oct 10, 1995

8/544381

Maureen T. Cronin - Los Altos CA
Charles Garrett Miyada - San Jose CA
Earl A. Hubbell - Mountain View CA
Mark Chee - Palo Alto CA
Stephen P. A. Fodor - Palo Alto CA
Xiaohua C. Huang - Mountain View CA
Robert J. Lipshutz - Palo Alto CA
Peter E. Lobban - Palo Alto CA
Macdonald S. Morris - Felton CA
Edward L. Sheldon - San Diego CA

Affymetrix, Inc. - Santa Clara CA

C12Q 168

435 6

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.

2004007, Apr 22, 2004

Oct 14, 2003

10/686440

Donald Ackley - Cardiff CA, US
Edward Sheldon - San Diego CA, US
Michael Krihak - San Diego CA, US

Nanogen, Inc. - San Diego CA

C12M003/00

435/287200, 435/288500

A device includes an inlet for receipt of a sample. A first chamber is coupled to the inlet and includes at least one affinity region. A second chamber is disposed adjacent to the first chamber. The first chamber and the second chamber share a common intermediate member, the intermediate member having at least one via formed in the common intermediate member. The second chamber includes an assay chip comprising an array of addressable electrodes. An outlet is coupled to the second chamber. The device may be used to selectively amplify and elute nucleic acids for subsequent detection and analysis.