DOUGLAS HOWARD JONES
Medical Practice in Iowa City, IA

6190889, Feb 20, 2001

Jan 7, 1999

9/226683

Douglas H. Jones - Iowa City IA

University of Iowa Research Foundation - Iowa City IA

C12P 1934

435 911

An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.

6368791, Apr 9, 2002

Feb 19, 1998

09/026033

Carolyn A. Felix - Ardmore PA
Douglas H. Jones - Iowa City IA
Eric Rappaport - Blackwood NJ

The Childrens Hospital of Philadelphia - Philadelphia PA

C12Q 168

435 6, 435 911, 435 912

The invention relates to kits and methods for panhandle PCR amplification of a region of DNA having an unknown nucleotide sequence, wherein the region flanks a region of a leukemia-associated gene having a known nucleotide sequence in a human patient. Amplification of an unknown region flanking a known region of a leukemia-associated gene permits identification of a translocation partner of the gene or identification of a duplicated sequence within the gene. The invention further relates to kits useful for performing the methods of the invention, to an isolated polynucleotide, and to primers derived from such an isolated polynucleotide.

5470722, Nov 28, 1995

May 6, 1993

8/058907

Douglas H. Jones - Iowa City IA

University of Iowa Research Foundation - Oakdale IA

C12N 1570, C12Q 168

435 912

A method that permits the rapid amplification of unknown DNA that flanks a known site, such that one can walk into an uncharacterized region of DNA. In this method, human genomic DNA is restriction enzyme digested and then ligated to a 5' phosphorylated-oligonucleotide so that the 5' end of each strand of genomic DNA is extended and phosphorylated. The phosphorylated-oligonucleotide is constructed to render 5' end extensions that are complementary to the known sequence. Following denaturation and re-annealing under dilute conditions that promote intrastrand annealing and under high stringency, only those DNA strands containing the known sequence will form a stem-loop structure with a recessed and phosphorylated 5' end, rendering a substrate for a subsequent heat-stable ligation reaction to another oligonucleotide. This second oligonucleotide is complementary to the sequence immediately adjacent to the phosphorylated-oligonucleotide high stringency annealing site. The heat-stable ligation reaction appends a known sequence to the DNA segments containing the two known contiguous DNA sequences used for oligonucleotide annealing.

5286632, Feb 15, 1994

Jan 9, 1991

7/638512

Douglas H. Jones - Iowa City IA

C12N 1510, C12N 1500, C12P 1934

435 912

The subject invention relates to a method referred to as recombination PCR (RPCR). In the method, the polymerase chain reaction is utilized to add double-stranded homologous ends to DNA. These homologous ends undergo recombination in vivo following transfection of host cells. The placement of these homologous ends, by the amplifying primers permits the rapid cloning of the desired mutant or recombinant, with a minimal number of steps and primers.

7134597, Nov 14, 2006

Aug 23, 2002

10/227159

Douglas W. Jones - Iowa City IA, US

University of Iowa Research Foundation - Iowa City IA

G06K 17/00

235386, 235375

A voting system that allows voters who may be blind or otherwise disabled to use election ballots without needing to see the ballot. The present invention provides a balloting system which presents and holds an election ballot while allowing a voter to cast and inspect votes. The system provides audible or tactile feedback when voting targets are probed with a wand or other pointing means. The feedback can comprise information pertaining to a candidate at a position on the ballot. The feedback can also comprise information pertaining to a mark that may or may not have been made on the ballot.

2003004, Mar 6, 2003

Apr 17, 2001

09/837621

Douglas Jones - Iowa City IA, US

University of Iowa Research Foundation

C12Q001/68, C12P019/34

435/006000, 435/091200

An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.

5411875, May 2, 1995

Nov 1, 1991

7/786902

Douglas H. Jones - Iowa City IA

University of Iowa Research Foundation - Oakdale IA

C12P 1934

435 912

A method that permits the highly specific PCR amplification of unknown DNA that flanks a known sequence region. In this method, known DNA is placed on the uncharacterized side of that specific sequence that contains the unknown DNA by a DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the segment of interest.

6258533, Jul 10, 2001

Mar 5, 1998

9/035183

Douglas H. Jones - Iowa City IA

The University of Iowa Research Foundation - Iowa City IA

C12Q 168, C12P 1934

435 6

An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.