DONALD B RISING
Engineering in Stow, MA

6750039, Jun 15, 2004

Mar 19, 2002

10/101026

Frederick G. Bargoot - Wellesley MA
Donald B. Rising - Stow MA
Henrik Stender - Waltham MA

Boston Probes, Inc. - Bedford MA

C12Q 104

435 34, 4352831, 4352865, 422 681

This invention relates to a filtration apparatus and method for the separation of microscopic entities from a fluid (liquid or gas) and subsequent visual or imaging microscopic analysis of the entities separated thereon either directly or after treatment of the entities whilst on the apparatus in order to enhance their subsequent visualization and/or imaging. Such treatment can comprise reaction with reagents contained in other solutions that can be made to imbibe and/or pass through the filtration apparatus and which can be washed with solutions that can be made to imbibe and/or pass through the filtration apparatus. An example of a specific area of application is in the microbiological testing of fluids in order to detect, identify and/or enumerate microorganisms contained in a fluid test sample.

5554536, Sep 10, 1996

Jan 5, 1995

8/369212

Donald B. Rising - Stow MA

Millipore Investment Holdings Limited - Wilmington DE

C12M 306

4353051

A device for conducting biological analysis, such as cell culture, is disclosed having improved contamination prevention features. In a preferred embodiment, a microwell membrane plate for cell analysis is adapted to be inserted into a shallow rectangular tray with elevated side walls which form a single large reservoir for holding an appropriate liquid used in cell growth and analysis. The side surfaces of the tray include a raised ridge extending about the entire periphery of the tray. A series of uniform-height steps formed on the ridge serve as support for the microwell membrane plate when inserted into the tray and also create a narrow capillary gap between the plate and the tray. A ledge extending laterally from the base of the ridge forms a second, wider gap when the microwell membrane plate is inserted into the tray. The height of the first capillary gap is less than the height of the second gap formed within the zone of space extending to the exterior of the device, thus when the tray and plate are tilted, as when jostled during handling, liquid from the reservoir will preferentially remain in the space formed by the first capillary gap.

2006004, Feb 23, 2006

Aug 23, 2005

11/209410

Phillip Clark - Wakefield MA, US
Chris Scott - Westford MA, US
Marc Emerick - Winchester MA, US
William Kopaciewicz - West Newbury MA, US
Donald Rising - Stow MA, US

G01N 31/00

436008000

An integrated proteomics sample preparation device and method for in-gel digestion of proteins and for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device and method of the present invention allow for digestion, desalting and concentration of sample prior to analysis. More specifically, the device in accordance with an embodiment of the present invention includes a plurality of wells in fluid communication with a an outlet or drainage opening containing a three dimensional structure comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. In a preferred embodiment, the wells are configured so as to prevent a sample carrier present in the wells from clogging the outlet when subjected to a driving force such as vacuum. The device also reduces or eliminates overflowing of a well in the event a drain becomes clogged during automated operation.

4966667, Oct 30, 1990

Apr 18, 1989

7/340107

Donald B. Rising - Stow MA
Rita Zimmerman - West Roxbury MA

Millipore Corporation - Bedford MA

G01N 2726, B01D 5702

2041821

An apparatus and process are provided for transferring a gel shaped as a cylinder containing a treated sample from a first step to a second electrophoresis step without distorting the position of the treated sample within the shaped gel. A shaped gel useful in electrophoresis is formed in a tube with a multifilament thread extending the entire length of and within the central portion of the shaped gel. The shaped gel then is employed in a first treatment step to form a treated sample that may be a separated sample. After extrusion of the gel from the tube, the portion of the thread extending from each end of the shaped gel is grasped and the shaped gel containing the treated sample is transferred to contact a slab gel along the entire length of the shaped gel to effect an electrophoresis step in the second treatment step.

7135148, Nov 14, 2006

Jun 11, 2002

10/166871

Kenneth DeSilets - Westford MA, US
Donald Rising - Stow MA, US
Jeanne Phillips - Littleton MA, US

Millipore Corporation - Billerica MA

B01L 11/00

422101, 422 99, 422100, 422102, 4352851, 436180

A multiwell filter plate of a multiwell test apparatus is provided to effect addition or removal of liquid from a feeding tray positioned below the multiwell filter tray. Access holes extending through a plate of the multiwell filter plate are provided so that a syringe can be extended through the access holes.

4974462, Dec 4, 1990

Mar 17, 1988

7/169523

Donald B. Rising - Stow MA
Emile O. Montminy - Lowell MA

Millipore Corporation - Bedford MA

G01N 104

73864041

A filter punch construction is provided for use in conjunction with a multiwell filtration device wherein each well includes a filtration membrane. The punch has a leading flat surface to which is secured an elongated piercing member having a sharp point and a following surface to which is secured a guide member. The piercing member pierces the filtration membrane in each well and the flat leading surface pushes the entire membrane and retentate thereon into a receptacle vial.

7018588, Mar 28, 2006

Jun 11, 2002

10/167250

Kenneth DeSilets - Westford MA, US
Jeanne Phillips - Littleton MA, US
Donald B. Rising - Stow MA, US

Millipore Corporation - Billerica MA

B01L 11/00

422101, 422 99, 422100, 422102, 436180

A multiwell test apparatus formed of a multiwell filter plate having a plurality of wells and a first feeding plate and a second receiver plate is provided. The wells of the multiwell filter plate each have an open bottom surface to which a membrane is attached, at least one first access hole providing access to the feeding/receiver plate below, a pair of alignment pins designed to fit alignment holes in the feeding/receiver plates in a particular arrangement. The first feeding plate is a single well plate or a multiwell plate used solely for feeding. The second receiver plate is a multiwell plate having the same number of wells as the filter plate and aligned so as to receive the wells of the filter plate therein used as the assaying plate. The use of alignment holes in the feeding/receiver plates and alignment posts in the filter plate ensure precise alignment of the plates when together.

6325096, Dec 4, 2001

Oct 25, 1999

9/426044

Donald Rising - Stow MA
John F. Zettler - Stow MA

Stargaze Corporation - Braintree MA

F16K 300, G05D 706

1374542

A variable orifice valve for controlling fluid flow in a conduit, comprising a band or other movable members defining an opening for passing the fluid therethrough, the band mounted transverse to the direction of fluid flow in the conduit, so that the band opening is at least partially in the conduit. The band is movable relative to the conduit, to change the amount of the opening exposed to fluid flow.

5326533, Jul 5, 1994

Nov 4, 1992

7/971498

Carolyn Lee - Sudbury MA
Donald Rising - Stow MA
Thomas Zermani - Concord MA

Millipore Corporation - Bedford MA

B01D 2708, B01D 2903, C12M 112, C12M 120

422101

A dialysis apparatus is provided including a multiwell plate, a separations membrane and a template having holes which mate with the wells. The membrane is sealed to one end of the wells by positioning the template about the outer peripheral surfaces of the wells with the membrane being frictionally held therebetween.

4525182, Jun 25, 1985

Aug 29, 1983

6/527546

Donald B. Rising - Stow MA
Richard G. Naegeli - Bedford MA

Millipore Corporation - Bedford MA

B01D 1900

55159

A filter apparatus is provided which is adapted for filtration of intravenous liquids and separates gas in the incoming intravenous liquid prior to delivering the filtered liquid to a patient. Incoming liquid is passed into a space within a filter holder, which space has a smaller portion that excludes gas due to surface tension forces and a larger portion where gas accumulates. A hydrophobic filter membrane is positioned adjacent the large portion of the space to permit passage of gas from the space. A hydrophilic filter membrane is positioned within the space to filter the liquid prior to administration to the patient.