Inventors:
RICHARD A. ROBISON - PROVO UT, US
DAVID O. PICKETT - KNOXVILLE TN, US
BEN SATTERFIELD - WEBSTER TX, US
International Classification:
C40B 30/04, C07H 21/04, C12Q 1/68
US Classification:
506 9, 435 611, 435 615, 536 2433, 536 2432
Abstract:
Provided herein is a method for detecting the presence or absence of at least one of toxin gene A, B, E, and F in a biological sample by means of PCR amplification using toxin specific primers and labeled probes in connection with real time or delayed detection. Also provided are specific primer and probe sequences, a diagnostic method and a kit comprising primers and probes for detection of toxin genes A, B, E, or F in a biological sample.