Inventors:
David N. Fredricks - Seattle WA, US
Prasanna D. Khot - Mountlake Terrace WA, US
Daisy L. Ko - Seattle WA, US
Assignee:
Fred Hutchinson Cancer Research Center - Seattle WA
International Classification:
C12Q 1/68, C07H 21/00
Abstract:
Disclosed herein are methods for detecting a fungal pathogen in a patient sample, involving isolating the sample, carrying out a PCR reaction on the sample to generate an amplicon that includes a region of the fungal 28S ribosomal RNA gene, and detecting the PCR amplicon. Also disclosed are sequences of primers for specifically detecting a broad range of fungal pathogens in the presence of human ribosomal DNA. In certain embodiments, the amplicon is detected by sequencing or by two-dimensional melt-curve analysis. In yet other embodiments, more than one fungal pathogen is detected in a sample using the methods disclosed herein.