DAVID MICHAEL LONG
Medical Practice in Boulder, CO
License number
Pennsylvania MD024482E
Category
Medicine
Type
Medical Physician and Surgeon
Address
Address
2
Boulder, CO 80303
Pennsylvania
Pennsylvania
Personal information
See more information about DAVID MICHAEL LONG at radaris.comName
Address
Phone
503 Westtown Cir, West Chester, PA 19382
506 Grant Ave, Croydon, PA 19021
4 Holly Dr, New Cumberlnd, PA 17070
50 Lincoln St, Exeter, PA 18643
519 Shuman St, Catawissa, PA 17820
Professional information
Ligation With Hammerhead Ribozymes
US Patent:
5633133, May 27, 1997
Filed:
Jul 14, 1994
Appl. No.:
8/275156
Inventors:
David M. Long - Boulder CO
Olke C. Uhlenbeck - Boulder CO
Klemens J. Hertel - Medford MA
Olke C. Uhlenbeck - Boulder CO
Klemens J. Hertel - Medford MA
International Classification:
C12Q 168, C12N 1511, C07H 2104
US Classification:
435 6
Abstract:
The claimed invention is drawn to a method for ligation of an enzymatic nucleic acid molecule having a hammerhead motif to a separate nucleic acid molecule. The method includes use of ligation enhancing conditions in which ligation is enhanced at least two fold over that observed in a medium containing only Mg. sup. 2+ as a divalent cation at 20. degree. C.