Inventors:
Richard A. Gatti - Sherman Oaks CA, US
Helen H. Chun - Woodland Hills CA, US
David J. Rawlings - Seattle WA, US
Assignee:
The Regents of the University of California - Oakland CA
International Classification:
G01N 33/53, C12P 21/06, C12N 15/00, C12N 5/08, A61K 39/12
US Classification:
435 71, 435 691, 4353201, 435372, 435367, 435456, 435183, 424 934, 424 9321, 4241991, 530350
Abstract:
The present disclosure concerns methods for recombinantly producing functional ataxia-telangiectasia (ATM) protein, methods for isolating recombinant functional ATM protein, and uses of ATM protein. In particular, a method is disclosed for using a vaccinia virus vector to express ATM, and using immunoprecipitation or affinity tagging to isolate recombinant ATM.