DAVID J LIM, LICSW, MBA, MSW
Social Work at Galer St, Seattle, WA

2005000, Jan 6, 2005

May 14, 2004

10/846147

Daniel Chiu - Seattle WA, US
Jason Kuo - Seattle WA, US
David Lim - Seattle WA, US

C12Q001/68, C12M001/34

435006000, 435287200

Embodiments of the present invention employ complexly shaped, high-surface-area channels for separation and purification of molecules, including important biopolymers such as proteins, glycoproteins, polysaccharides, and other molecular components of living cells. The relatively large internal surface areas of the complexly shaped channels employed in embodiments of the present invention provide, in comparison to traditional, simply shaped separation channels, increased heat dissipation during electrokinetic separation, and a decreased tendency for bulk-solution flow. Heat dissipation prevents high temperatures that can denature proteins and that can induce thermal currents within the separation channel. Bulk-solution flow within a separation channel can overwhelm the generally linear, electrical-potential-induced migration of molecules that leads to efficient and well-resolved molecular separations. The complexly shaped channels employed in various embodiments of the present invention can be readily manufactured at microscale dimensions for use in microscale devices, at millimeter-scale dimensions for inclusion in microfluidics devices, and may also be used in larger scale, traditional separation and purification systems.

2010032, Dec 23, 2010

Jun 12, 2008

12/665575

Daniel T. Chiu - Seattle WA, US
Jason S. Kuo - Seattle WA, US
J. Patrick Shelby - Bellevue WA, US
David S. Lim - Seattle WA, US

University of Washington - Box 354990, Seattle WA

C12Q 1/02, C12N 5/02, C12M 1/00

435 29, 435325, 4352831

Embodiments in accordance with the present invention relate to the use of effusive filtration to segregate tumor cells from a sample of bodily fluid. In one embodiment, fluid containing a cell is flowed down a channel having a filtration medium present along at least one side wall. The tumor cell is captured when the fluid passes through the filtration medium. Accumulated pressure on the captured tumor cell is reduced by allowing the fluid that has passed through the filtration medium to re-enter the channel. In a particular embodiment, the filtration medium may comprise side wall apertures having a width smaller than that of the cell, with downstream apertures allowing re-entry of the fluid into the channel.

2008031, Dec 25, 2008

Jun 20, 2007

11/766053

Daniel T. Chiu - Seattle WA, US
Jason S. Kuo - Seattle WA, US
J. Patrick Shelby - Seattle WA, US
David S.W. Lim - Seattle WA, US

University of Washington - Seattle WA

G01N 33/49, B01D 29/05, B01D 37/00

436 64, 210194, 210196, 210435, 210483, 210749, 210767, 210805

Embodiments in accordance with the present invention relate to the use of effusive filtration to segregate tumor cells from a sample of bodily fluid. In one embodiment, fluid containing a cell is flowed down a channel having a filtration medium present along at least one side wall. The tumor cell is captured when the fluid passes through the filtration medium. Accumulated pressure on the captured tumor cell is reduced by allowing the fluid that has passed through the filtration medium to re-enter the channel. In a particular embodiment, the filtration medium may comprise side wall apertures having a width smaller than that of the cell, with downstream apertures allowing re-entry of the fluid into the channel.

2005004, Mar 3, 2005

Aug 25, 2004

10/926656

Daniel Chiu - Seattle WA, US
Bingyun Sun - Seattle WA, US
James Shelby - Bellevue WA, US
John Edgar - Seattle WA, US
Gavin Jeffries - Seattle WA, US
Robert Lorenz - Seattle WA, US
Jason Kuo - Seattle WA, US
Mingyan He - Lynnwood WA, US
Peter Allen - Seattle WA, US
Sarah Mutch - Seattle WA, US
Christopher Kuyper - Seattle WA, US
Gina Fiorini - Redmond WA, US
David Lim - Seattle WA, US

G01N033/53, C12M001/34

435007100, 435287200

A method and system for performing biochemical detection or analysis on micro- and nano-scale subcellular component within a single biological cell is provided. An integrated platform device and method to perform the biochemical analysis is also provided.

2004008, May 6, 2004

Mar 17, 2003

10/391547

Karen Fleckner - Tacoma WA, US
Feng Zheng - Seattle WA, US
Cynthia Buenviaje - Seattle WA, US
Yao Huang - Vancouver WA, US
Jeff Pedersen - Tacoma WA, US
David Lim - Seattle WA, US
H. Fuji - Seattle WA, US
Jeremy Hergesheimer - Seattle WA, US
Michael Treiber - Seattle WA, US

H01M008/02, H01M008/24, H01M008/10, H01M004/96, H01M004/92, H01M008/04, H01M004/88, B05D005/12

429/038000, 429/030000, 429/044000, 429/032000, 429/022000, 429/025000, 429/033000, 502/101000, 427/115000

A novel design and process for: (a) a membrane electrode assembly (MEA) with aligned carbon nanotubes as a nano-scale gas distrubutor which yield better gas conversion efficiencies in PEM fuel cells, and (b) doped silicon flow field plates (FFP) which increase electrode conductivity of the membrane-catalyst-gas diffusion layer (GDL)-FFP interfaces of the proton exchange membrane fuel cell (PEMFC). Also, part of the invention are a stacking configuration and a gas distribution design that also enhance conductivity of carbon/metal catalyst/electrode, GDL, and FFP interface surfaces without crushing the FFPs. Aligned carbon nanoscale gas distributors are employed at the interfaces, thereby increasing the overall performance of th PEMFC. The FFPs are easy to manufacture and mass-producible, yet mechanically sturdy and significantly lighter in weight than their conventional counterparts. Another novel feature of the invention is an integrated monitoring and communication/Internet system located directly or connected to the FFP.