David C. Wright
General Contractor in Chapel Hill, NC

7223536, May 29, 2007

Feb 7, 2001

09/778168

David J. Wright - Chapel Hill NC, US
Maria A. Milla - Wynnewood PA, US
James G. Nadeau - Chapel Hill NC, US
G. Terrance Walker - Chapel Hill NC, US

Becton, Dickinson and Company - Franklin Lakes NJ

C12Q 1/68, C12P 19/34, C07H 21/04

435 6, 435 912, 536 231, 536 243

The present invention provides methods for detecting and identifying sequence variations in a nucleic acid sequence of interest using a detector primer. It has been found that the reduced efficiency of primer extension by DNA polymerases when the 3′ end of a primer does not hybridize perfectly with the target can be adapted for use as a means for distinguishing or identifying the nucleotide in the target which is at the site where the diagnostic mismatch between the detector primer and the target occurs. The detector primer hybridizes to the sequence of interest and is extended with polymerase. The efficiency of detector primer extension is detected as an indication of the presence and/or identity of the sequence variation in the target. The inventive methods make use of nucleotide mismatches at or near the 3′ end of the detector primer to discriminate between the nucleotide sequence of interest and a second nucleotide sequence which may occur at that same site in the target. The methods are particularly well suited for detecting and identifying single nucleotide differences between a target sequence of interest (e. g.

2001003, Nov 8, 2001

Feb 7, 2001

09/778175

David Wright - Chapel Hill NC, US
Maria Milla - Wynnewood PA, US
James Nadeau - Chapel Hill NC, US
G. Walker - Chapel Hill NC, US

C12P019/34, C07H021/04, C12Q001/68

536/023100, 435/006000, 435/091100

The present invention provides methods for detecting and identifying sequence variations in a nucleic acid sequence of interest using a detector primer. It has been found that the reduced efficiency of primer extension by DNA polymerases when the 3′ end of a primer does not hybridize perfectly with the target can be adapted for use as a means for distinguishing or identifying the nucleotide in the target which is at the site where the diagnostic mismatch between the detector primer and the target occurs. The detector primer hybridizes to the sequence of interest and is extended with polymerase. The efficiency of detector primer extension is detected as an indication of the presence and/or identity of the sequence variation in the target. The inventive methods make use of nucleotide mismatches at or near the 3′ end of the detector primer to discriminate between the nucleotide sequence of interest and a second nucleotide sequence which may occur at that same site in the target. The methods are particularly well suited for detecting and identifying single nucleotide differences between a target sequence of interest (e.g., a mutant allele of a gene) and a second nucleic acid sequence (e.g., a wild type allele for the same gene).

2002002, Feb 28, 2002

Jun 17, 1999

09/335218

DAVID J. WRIGHT - CHAPEL HILL NC, US
MARIA MILLA - WYNNEWOOD PA, US
JAMES G. NADEAU - CHAPEL HILL NC, US
G. TERRANCE WALKER - CHAPEL HILL NC, US

C12Q001/68, C07H021/04, C12P019/34

435/006000, 536/024300, 435/091200

The present invention provides methods for detecting and identifying sequence variations in a nucleic acid sequence of interest using a detector primer. It has been found that the reduced efficiency of primer extension by DNA polymerases when the 3′ end of a primer does not hybridize perfectly with the target can be adapted for use as a means for distinguishing or identifying the nucleotide in the target which is at the site where the diagnostic mismatch between the detector primer and the target occurs. The detector primer hybridizes to the sequence of interest and is extended with polymerase. The efficiency of detector primer extension is detected as an indication of the presence and/or identity of the sequence variation in the target. The inventive methods make use of nucleotide mismatches at or near the 3′ end of the detector primer to discriminate between the nucleotide sequence of interest and a second nucleotide sequence which may occur at that same site in the target. The methods are particularly well suited for detecting and identifying single nucleotide differences between a target sequence of interest (e.g., a mutant allele of a gene) and a second nucleic acid sequence (e.g., a wild type allele for the same gene).

5811269, Sep 22, 1998

Apr 30, 1996

8/640378

James G. Nadeau - Chapel Hill NC
Cheryl H. Dean - Raleigh NC
James L. Schram - Knightdale NC
Deborah R. Howard - Durham NC
Margaret S. Dey - Durham NC
David J. Wright - Chapel Hill NC

Becton, Dickinson and Company - Franklin Lakes NJ

C12P 1934, C07H 2104

435 911

Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of the Mycobacterium tuberculosis (M. tb) complex and a 16S rDNA target common to essentially all mycobacteria are described. In certain embodiments, the primers are optimized for efficient multiplex amplification in thermophilic SDA. The multiplex Strand Displacement Amplification methods of the invention are capable, in a single amplification reaction, of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of mycobacteria. Also disclosed are internal control sequences designed for coamplification with the two targets, allowing assessment of amplification efficiency and/or quantitation of the targets.

5648211, Jul 15, 1997

Apr 18, 1994

8/229279

Melinda S. Fraiser - Durham NC
Catherine A. Spargo - Cary NC
George Terrance Walker - Chapel Hill NC
Mark Van Cleve - San Jose CA
David James Wright - Chapel Hill NC
Michael C. Little - Baltimore MD

Becton, Dickinson and Company - Franklin Lakes NJ

C12Q 168, C12P 1934

435 6

Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37. degree. C. to 70. degree. C. ). The preferred temperature range for thermophilic SDA is 50. degree. C. to 70. degree. C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products. In addition, the need to add the enzymes in a separate step after the initial heat denaturation of double stranded targets is eliminated when enzymes capable of tolerating the denaturation temperature are used.

6855556, Feb 15, 2005

Jan 4, 2002

10/040077

Terry J. Amiss - Cary NC, US
Colleen M. Nycz - Raleigh NC, US
J. Bruce Pitner - Durham NC, US
Douglas B. Sherman - Durham NC, US
David J. Wright - Chapel Hill NC, US

Becton, Dickinson and Company - Franklin Lakes NJ

G01N033/48

436 95, 438 86, 438164, 438172, 435 72, 435 14, 4352871, 435817

The invention is directed to compositions of mutated binding proteins containing reporter groups, analyte biosensor devices derived there from, and their use as analyte biosensor both in vitro and in vivo.