Inventors:
Vladimir Didenko - Houston TX, US
David Baskin - Houston TX, US
Assignee:
Baylor College of Medicine - Houston TX
International Classification:
C12Q001/68, G01N001/30, G01N033/48, C07H021/02, C07H021/04
US Classification:
435 6, 435 405, 536 231, 536 243
Abstract:
Several types of DNA cuts are used as markers of apoptosis for detection of apoptotic cells in situ. The present invention includes a method to detect DNase II type DNA damage, bearing 5′OH using vaccinia topoisomerase I. The present invention also includes a method combining a ligase-based method to detect DNase I type DNA damage and the topoisomerase based method, resulting in simultaneous detection of two specific types of DNA damage in situ.