DR. DAVID A. HAAKE, M.D.
Osteopathic Medicine in Los Angeles, CA

6699482, Mar 2, 2004

Nov 13, 2001

09/992807

David A. Haake - Culver City CA
Ellen S. Shang - Los Angeles CA

The Regents of the University of California - Oakland CA

A61K 3902

4242341, 4241901, 435 691, 435 693, 435 711, 530300, 530350, 536 237

The present invention presents novel leptospiral membrane lipoproteins, LipL1 and LipL2, associated with pathogenic strains of Leptospira. LipL1 is of about 35 kDa, and LipL2 is of about 41 kDa. Also disclosed are the method for purifying these proteins from Leptospira, their nucleotide and amino acid sequences, the cloning of the genes encoding the proteins and their recombinant proteins, methods for producing antibodies to these proteins, the resulting antibodies. These proteins, their immunogenic fragments, and antibodies against them, are useful for inducing an immune response to pathogenic Leptospira as well as providing a diagnostic target for leptospirosis.

6685945, Feb 3, 2004

Aug 19, 1997

08/914350

David A. Haake - Culver City CA
David R. Blanco - Beverly Hills CA
Cheryl I. Champion - Culver City CA
Michael A. Lovett - Los Angeles CA
James N. Miller - Northridge CA

The Regents of the University of California - Oakland CA

A61K 3902

4241901, 4242341, 530350, 530825, 430200

An antigenic preparation is provided which contains a 31 Kd outer membrane protein from Leptospira which can be used immunologically as a vaccine for leptospirosis caused by this organism.

2011002, Feb 3, 2011

May 26, 2010

12/788101

DAVID A. HAAKE - CULVER CITY CA, US
BERNARD M. CHURCHILL - PACIFIC PALISADES CA, US
JOSEPH C. LIAO - LOS ALTOS CA, US
MARC A. SUCHARD - LOS ANGELES CA, US
YANG LI - LOS ANGELES CA, US
MITRA MASTALI - MARINA DEL REY CA, US

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - OAKLAND CA
U.S. GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS - WASHINGTON DC

C12Q 1/68, C07H 21/04

435 6, 536 243

Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.

6306623, Oct 23, 2001

Feb 24, 1998

9/028586

David A. Haake - Culver City CA

The University of California - Oakland CA

C12N 1509, C12N 120, C12N 1500, C12N 500, C07H 2102

435 693

An antigenic preparation is provided containing an outer membrane protein associated with pathogenic strains of Leptospira. The protein has been designated "LipL32" for "lipoprotein from Leptospira" and because the isolated polypeptide migrates to a position corresponding to a molecular weight of 32 kD in a denaturing polyacrylamide gel. The invention provides polynucleotides encoding LipL32 and antibodies that bind the protein which are useful in the diagnosis of leptospirosis. In addition, LipL32 can be used immunologically as a vaccine for spirochete-associated pathologies.

5558993, Sep 24, 1996

Jun 17, 1994

8/261825

Cheryl I. Champion - Culver City CA
Michael A. Lovett - Los Angeles CA
David A. Haake - Culver City CA
James N. Miller - Northridge CA
David R. Blanco - Northridge CA

The Regents of the University of California - Oakland CA

C07H 2102, C07H 2104, C12N 121, C12N 1570, C12P 2100, C12Q 168

435 6

A polynucleotide encoding a 17-kD virulence protein, called EppA, from Borrelia burgdorferi is provided. The protein encoded by the polynucleotide of the invention is useful immunologically as a vaccine for Lyme borreliosis caused by B. burgdorferi. Methods and kits for detection of EppA polynucleotide are also provided.

6140083, Oct 31, 2000

Jul 23, 1998

9/122210

David A. Haake - Culver City CA

The Regents of the University of California - Oakland CA

C12P 2106

435 693

An antigenic preparation is provided containing an outer membrane protein associated with pathogenic strains of Leptospira. The protein has been designated "LipL46" for "lipoprotein from Leptospira" and because the isolated polypeptide migrates to a position corresponding to a molecular weight of 46 kD in a denaturing polyacrylamide gel. The invention provides polynucleotides encoding LipL46 and antibodies that bind the protein which are useful in the diagnosis of leptospirosis. In addition, LipL46 can be used immunologically as a vaccine for spirochete-associated pathologies.

5824321, Oct 20, 1998

Jul 1, 1997

8/886863

David A. Haake - Culver City CA

The Regents of the University of California - Oakland CA

A61K 39002

4242661

An antigenic preparation is provided which contains a 63 Kd outer membrane protein from Leptospira which can be used immunologically as a vaccine for leptospirosis caused by this organism. Also provided in the invention are polynucleotides encoding the protein and antibodies which bind the protein which are useful in the diagnosis of leptospirosis.

8216594, Jul 10, 2012

Jan 26, 2012

13/359354

Albert I. Ko - Bahia, BR
Mitermayer Galvão Reis - Bahia, BR
Julio Henrique Rosa Croda - Bahia, BR
Isadora Cristina Siqueira - Bahia, BR
David A. Haake - Los Angeles CA, US
James Matsunaga - Los Angeles CA, US
Lee W. Riley - Berkeley CA, US
Michele Barocchi - Florence, IT
Tracy Ann Young - Oakland CA, US

The United States of America as represented by the Department of Veterans Affairs - Washington DC
The Regents of the University of California - Oakland CA
Cornell Research Foundation, Inc. - Ithaca NY

A61K 39/002

4242691, 4241911, 530300, 530350

The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

5854395, Dec 29, 1998

Sep 24, 1996

8/719124

Cheryl I. Champion - Culver City CA
Michael A. Lovett - Los Angeles CA
David A. Haake - Culver City CA
James N. Miller - Northridge CA
David R. Blanco - Beverly Hills CA

The Regents of the University of California - Oakland CA

C07K 14195

530350

The present invention provides EppA polypeptide, a Borrelia burgdorferi virulence protein. This 17-kD outer membrane protein has been designated EppA for exported plasmid protein A.