DAVID A DEFRANCO
Water and Wastewater in Lynn, MA

Veolia Water
Senior Laboratory Technician, City of Lynn Water Treatment Plant Pfizer/Wyeth - Andover, MA
Senior Associate Scientist (Research Scientist) Wyeth - Andover, MA
Scientist II, Protein Pharmacokinetics ImmunoGen, Inc - Cambridge, MA
Research Associate II, Assay Development Eastern Ambulance - Woburn, MA
Emergency Medical Technician Patriot Ambulance - Lowell, MA
Emergency Medical Technician Beth Israel Deaconess Medical Center - Boston, MA
Research Associate Tufts University Medical School - Boston, MA
Senior Research Technician

University Of California - San Diego - San Diego, CA
Graduate Certificate in Biostatistics University Of Massachusetts - Lowell, MA
Bachelors of Science in Biology

ligand-binding assay development (ELISA, MSD Sector Imager, Gyrolab), radiolabeling of proteins and nucleic acids, cell-based assays, Western blot, eukaryotic tissue culture, hybridoma cultivation, host cell protein assays, radioimmunoassay, cAMP cellular assays, intracellular calcium flux assays, analysis of biological samples from radiodosed animals, cytotoxicity assay, RNA isolation from eukaryotic cell cultures, cDNA preparation, electrophoresis of nucleic acids and SDS-PAGE, densitometry, TLC, Ficoll Paque sedimentation, PCR, sequencing of Ig genes, small animal handling, protein purification, FACS analysis (with CellQuest software), Staclot, bacterial growth and transformation, plasmid preparation, HPLC, dosing formulation for in vivo studies, SAS programming, SOP generation, MS Excel, MS Word, Graphpad Prism, and Minitab