PROF. DANIEL WALTER NEBERT, M.D.
Medical Practice at Oliver Rd, Cincinnati, OH
License number
Ohio 35.064660
Category
Medical Practice
Type
Research Study
Address
Address
20 Oliver Rd, Cincinnati, OH 45215
Phone
(513) 821-4664
(513) 558-4897 (Fax)
(513) 558-4897 (Fax)
Personal information
See more information about DANIEL WALTER NEBERT at radaris.comName
Address
Phone
165 Burns Ave, Cincinnati, OH 45215
20 Oliver St, Wyoming, OH 45215
165 Burns Ave, Cincinnati, OH 45215
20 Oliver Rd, Cincinnati, OH 45215
410 Poplar Ave, Cincinnati, OH 45215
Professional information
Dr. Daniel W Nebert, Cincinnati OH - MD (Doctor of Medicine)
Specialties:
Other, Pediatrics
Address:
20 Oliver Rd, Cincinnati 45215
(513) 821-4664 (Phone)
(513) 821-4664 (Phone)
Languages:
English
Education:
Medical School
Oregon Health Sciences University
Graduated: 1964
Oregon Health Sciences University
Graduated: 1964
Transgenic Animals For Monitoring Water Quality
US Patent:
2006014, Jun 29, 2006
Filed:
Nov 23, 2005
Appl. No.:
11/286613
Inventors:
Daniel Nebert - Cincinnati OH, US
International Classification:
A01K 67/027
US Classification:
800003000, 800020000
Abstract:
The present invention provides methods and systems that uses transgenic zebrafish with an easily assessable reporter gene under the control of pollutant-inducible DNA response elements. Transgenic zebrafish, carrying pollution-inducible response elements, are placed in the water to be tested, and the contaminants become bioconcentrated (generally 1,000- to 40,000-fold, relative to the water) in the tissues of the fish thereby activating specific response elements, which up-regulate the LUC reporter gene. Fish are then removed from the test water and placed immediately in a luminometer cuvette and incubated with luciferin. Luciferin is rapidly taken up into the tissues of the fish, oxidized by luciferase, and light is produced. The luminescence is proportional to the environmental concentration of the pollutant (to which the fish had been exposed), which drives the expression of the LUC gene by means of the various DNA motifs. The luminescence is quantitated in the luminometer. In each response element-containing construct, a specific class of polluting chemicals, allowing for differential identification of pollutants in a complex mixture activates the expression of the LUC gene. This assay does not require killing the fish and allows for repeated analysis of the same site with the same fish. The sensitivity of the system can be manipulated by varying the sequence of the response element.
Transgenic Animals For Monitoring Water Quality
US Patent:
2004014, Jul 29, 2004
Filed:
May 22, 2001
Appl. No.:
09/863528
Inventors:
Daniel Nebert - Cincinnati OH, US
International Classification:
G01N021/00
US Classification:
436/039000, 436/073000, 436/081000, 436/164000
Abstract:
The present invention provides methods and systems that uses transgenic zebrafish with an easily assessable reporter gene under the control of pollutant-inducible DNA response elements. Transgenic zebrafish, carrying pollution-inducible response elements, are placed in the water to be tested, and the contaminants become bioconcentrated (generally 1,000- to 40,000-fold, relative to the water) in the tissues of the fish thereby activating specific response elements, which up-regulate the LUC reporter gene. Fish are then removed from the test water and placed immediately in a luminometer cuvette and incubated with luciferin. Luciferin is rapidly taken up into the tissues of the fish, oxidized by luciferase, and light is produced. The luminescence is proportional to the environmental concentration of the pollutant (to which the fish had been exposed), which drives the expression of the LUC gene by means of the various DNA motifs. The luminescence is quantitated in the luminometer. In each response element-containing construct, a specific class of polluting chemicals, allowing for differential identification of pollutants in a complex mixture activates the expression of the LUC gene. This assay does not require killing the fish and allows for repeated analysis of the same site with the same fish. The sensitivity of the system can be manipulated by varying the sequence of the response element.