Daniel Robert Budman
Physician at Ryefield Rd, Matinecock, NY
License number
Colorado 38447
Issued Date
Feb 9, 2000
Renew Date
Jun 1, 2009
Expiration Date
May 31, 2011
Type
Physician
Address
Address
73 Ryefield Rd, Matinecock, NY 11560
Professional information
Identification Of Differentially Methylated Multiple Drug Resistance Loci
US Patent:
6994991, Feb 7, 2006
Filed:
Nov 18, 1999
Appl. No.:
09/441857
Inventors:
Li-ming Yuan - Flushing NY, US
Daniel Budman - Locust Valley NY, US
Anthony Calabro - Bethpage NY, US
Daniel Budman - Locust Valley NY, US
Anthony Calabro - Bethpage NY, US
Assignee:
North Shore - Long Island Jewish Research Institute - Manhasset NY
International Classification:
C12N 15/00, C12N 15/63, C12N 5/10, C07H 21/04
US Classification:
435 691, 4353201, 4352523, 43525411, 435325, 530 235
Abstract:
The present invention provides genomic loci which are hypermethylated and differentially expressed in drug resistant cells compared to non-drug resistant cells. These genomic loci are homologous to the rab6 locus but map to a different chromosomal position. The present invention also provides nucleic acids isolated from these genomic loci by Methyl-Differential Display (MDD) methods, including genomic DNAs and cDNAs. The present nucleic acids are useful as probes for detecting mutations and the methylation patterns of the newly identified genomic loci, and of homologous nucleic acids. Nucleic acids of the present invention are also useful for detecting expression of mRNA from herein identified genes, for measuring expression of those and homologous genes sequences, and for determining suitability of therapeutic treatment. The disclosed nucleic acids and their homologs are useful for inhibition of multiple drug resistance. Cells are disclosed which are useful for identification or modulators of multidrug resistance.