DANIEL LOUIS KACIAN
Medical Practice in San Diego, CA

5837452, Nov 17, 1998

Jan 3, 1997

8/778582

Kathleen A. Clark - Cardiff by the Sea CA
Daniel L. Kacian - San Diego CA

Gen-Probe Incorporated - San Diego CA

C12Q 168, C12N 100, C12N 106, B01F 1700

435 6

Methods for extracting nucleic acids by heating sample cells at about 80-95 degrees C. in a permeabilization reagent containing a non-ionic detergent and a metal chelating agent. The methods of the invention release large fragments of undegraded nucleic acids without physically disrupting the entire cell wall. The nucleic acids are released into solution without bursting the cells and are suitable for research and testing without further purification. The extraction method described herein is rapid, easy to perform, and applicable to a wide variety of cells, including microorganisms. Clinical samples may be screened for the presence or absence of a microorganism by heating the sample at 80-95 degrees Celsius in the presence of a non-ionic detergent and a metal chelating agent, adding to the sample a nucleic acid probe specific to the selected microorganism, incubating the sample under conditions which allow the probe to hybridize to released nucleic acid and detecting whether any hybridized probe is present. A kit for performing the nucleic acid extraction methods disclosed herein is also described.

5932450, Aug 3, 1999

Jun 7, 1995

8/484519

Nanibhushan Dattagupta - San Diego CA
Daniel L. Kacian - San Diego CA
David Bruce Lackey - San Diego CA

Gen-Probe Incorporated - San Diego CA

C12P 1934, C12Q 168, A01N 4304, C07H 2104

435 911

The present invention describes methods of synthesizing oligonucleotides. In particular, it relates to methods of enzymatically synthesizing chirally pure oligonucleotides using templates which can be digested after synthesis. By using digestible templates, separation and purification of the synthesized oligonucleotides are greatly facilitated.

5639599, Jun 17, 1997

Jun 6, 1994

8/254310

Thomas B. Ryder - Escondido CA
Daniel L. Kacian - San Diego CA

Gen-Probe Incorporated - San Diego CA

C12Q 170, C12Q 168, C12P 1934

435 5

Method and kit for the preparation of nucleic acid, such as for amplification. The nucleic acid may be prepared by centrifuging a sample of whole cells, particularly mononuclear cells, with an appropriate centrifugation medium so as to separate desired cells from non-desired cells, then removing and lysing said desired cells. A complexing agent able to complex ferric ion may be added to the lysed cells. The nucleic acid associated with the lysed cells can be amplified, and the nucleic acid can also be screened for a certain nucleic acid sequence, such as a vital nucleic acid sequence.

5612200, Mar 18, 1997

Mar 28, 1994

8/218990

Nanibhushan Dattagupta - San Diego CA
Thomas B. Ryder - Escondido CA
Daniel L. Kacian - San Diego CA

Gen-Probe Incorporated - San Diego CA

C12P 1934

435 912

Method for destroying the ability of a nucleic acid to be amplified, comprising the step of contacting the nucleic acid with bleach in an amount and for a time sufficient to inhibit the ability of that nucleic acid to be amplified in an amplification reaction.

7648680, Jan 19, 2010

Oct 26, 2004

10/973521

Bruce W. Anderson - Rancho Cucamonga CA, US
Nick M. Carter - Mooresville NC, US
Mordi I. Iheme - San Diego CA, US
Daniel L. Kacian - San Diego CA, US

Gen-Probe Incorporated - San Diego CA

B01L 3/00

422102

Method for obtaining a fluid sample from a collection device which includes assembling cap and fluid-holding vessel components of the collection device to contain and isolate a specimen retrieval device adjacent a side wall of the vessel. The position of the specimen retrieval device within the collection device is such that the specimen retrieval device does not substantially interfere with the movement of a fluid transfer device into or out of the assembled collection device. The fluid transfer device is used to draw and remove a fluid substance from the collection device for analysis.

RE38960, Jan 31, 2006

Jun 7, 2002

10/165281

Nanibhushan Dattagupta - San Diego CA, US
Paul Douglas Stull - San Diego CA, US
Marc Spingola - Albuquerque NM, US
Daniel Louis Kacian - San Diego CA, US

Gen-Probe Incorporated - San Diego CA

C12P 19/34, C12Q 1/68, C12M 1/34, C07H 21/02, C07H 21/04

435 912, 435 6, 435 911, 4352872, 536 231, 536 2433

Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.

2008014, Jun 26, 2008

Mar 7, 2008

12/044152

Mordi I. IHEME - San Diego CA, US
Daniel L. KACIAN - San Diego CA, US
Mark R. KENNEDY - South Burlington VT, US

GEN-PROBE INCORPORATED - San Diego CA

G01N 1/14

7386411

A method for accessing the contents of an assembly that includes a cap in sealing engagement with a fluid-holding vessel. In the method, one or more rib structures on an outer surface of a plastic pipette tip engage the cap as the pipette tip penetrates the cap and enters the assembly. A fluid transfer apparatus operationally associated with the pipette tip draws a fluid from the assembly into the pipette tip. The fluid-holding pipette tip is then removed from the assembly.

8211710, Jul 3, 2012

Oct 30, 2007

11/928984

Kathleen A. Dickey - Cardiff CA, US
Bruce W. Anderson - Las Vegas NV, US
Mordi I. Iheme - San Diego CA, US
Nick M. Carter - Elk Grove CA, US
Daniel L. Kacian - San Diego CA, US

G01N 1/10

436180, 422500, 422501, 422502, 436177, 215247, 215248, 215249, 215250

A method for obtaining the contents of a fluid-holding vessel having a cap secured to an open end thereof. The method includes penetrating a surface of the cap with a fluid transfer device and forming one or more air passageways between the penetrated surface of the cap and the fluid transfer device to allow air to be vented from the vessel. At least a portion of the contents of the vessel is then drawn into the fluid transfer device before the fluid transfer device is removed from the vessel.

5554516, Sep 10, 1996

Dec 2, 1993

8/162836

Daniel L. Kacian - San Diego CA
Diane L. McAllister - San Diego CA
Sherrol H. McDonough - San Diego CA
Nanibhushan Dattagupta - San Diego CA

Gen-Probe Incorporated - San Diego CA

C12P 1934, C12Q 168, C07H 2104

435 9121

A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reverse transcriptase and an RNA polymerase are provided to a mixture including the target sequence; the amplification proceeds accordingly. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

7691332, Apr 6, 2010

Oct 9, 2007

11/869233

Daniel L. Kacian - San Diego CA, US
Mark R. Kennedy - South Burlington VT, US
Nick M. Carter - Mooresville NC, US

Gen-Probe Incorporated - San Diego CA

B65D 41/20, B01L 3/02

422100, 422 99, 422101, 422102, 436177, 436180, 215247, 215248, 215249, 215250

A cap which can form an essentially leak-proof seal with an open-ended vessel capable of receiving and holding fluid specimens or other materials for analysis. To minimize potentially contaminating contact between a fluid sample present in the vessel and humans or the environment, the present invention features a cap having a frangible seal which is penetrable by a plastic pipette tip or other fluid transfer device. The cap further includes a filter for limiting dissemination of an aerosol or bubbles once the frangible seal has been pierced. The filter is positioned between the frangible seal and a retaining structure. The retaining structure is positioned on the cap above the filter and may be used to contain the filter within the cap. The material of the retaining structure may be penetrable by a fluid transfer device.