Inventors:
Joseph T. Bruder - Ijamsville MD, US
Imre Kovesdi - Rockville MD, US
Duncan L. McVey - Derwood MD, US
Douglas E. Brough - Gaithersburg MD, US
C. Richter King - Washington DC, US
Denise Louise Doolan - Camp Hill, AU
Joao Carlos Aguair - Potomac MD, US
Daniel John Carucci - Washington DC, US
Martha Sedegah - Gaithersburg MD, US
Walter R. Weiss - Bethesda MD, US
Keith Limbach - Gaithersburg MD, US
Assignee:
The United States of America as Represented by the Secretary of the Navy - Washington DC
International Classification:
C12Q 1/70, C12N 1/10, A61K 39/00, A61K 39/38, A61K 39/015, C40B 40/02, C40B 50/06
US Classification:
435 5, 4352582, 4241841, 4242681, 4242721, 506 14, 506 26
Abstract:
The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.