DANIEL ALAN KEYS
Pilots at Cresthaven, Irvine, CA
License number
California A3928080
Issued Date
Mar 2017
Expiration Date
Mar 2019
Category
Airmen
Type
Authorized Aircraft Instructor
Address
Address
8 Cresthaven, Irvine, CA 92604
Professional information
Oligonucleotide Probes For Detecting Nucleic Acids Through Changes In Flourescence Resonance Energy Transfer
US Patent:
6593091, Jul 15, 2003
Filed:
Sep 24, 2001
Appl. No.:
09/962040
Inventors:
Daniel A. Keys - Irvine CA
Firdous Farooqui - Brea CA
M. Parameswara Reddy - Brea CA
Firdous Farooqui - Brea CA
M. Parameswara Reddy - Brea CA
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
C12Q 168
US Classification:
435 6, 435 912
Abstract:
Oligonucleotide probes, kits, and methods useful for detecting a polynucleotide target in a sample are provided. The method, a mixture is formed by combining a polynucleotide target sample, a first probe that is complementary to the polynucleotide target and having a first fluorescent donor or fluorescent acceptor; and a second probe that is partially complementary to the first probe and having a second fluorescent donor or fluorescent acceptor. The second probe competes with the polynucleotide target for binding to the first probe and the first probe preferentially binds to the polynucleotide target rather than to the second probe. The-first fluorescent donor or acceptor and second fluorescent donor or acceptor form a donor/acceptor pair capable of fluorescence resonance energy transfer (FRET) with each other in response to activation of the fluorescent donor by light of a predetermined wavelength or band of wavelengths.
Method And Reagent For Suppressing The Injection Of Nucleic Acid Sequencing Template Into Micro-Bore Capillary During Dna Separation With Capillary Electrophoresis
US Patent:
6294064, Sep 25, 2001
Filed:
Nov 23, 1999
Appl. No.:
9/447386
Inventors:
M. Parameswara Reddy - Orange CA
Tung-Liang Huang - Placentia CA
Chitra K. Ratnayake - Yorba Linda CA
Daniel A. Keys - Irvine CA
Tung-Liang Huang - Placentia CA
Chitra K. Ratnayake - Yorba Linda CA
Daniel A. Keys - Irvine CA
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
B01D 5102, B01D 5942, G01N 3300, C07H 2104, C12P 1934
US Classification:
204451
Abstract:
Reagents and methods for preparing samples containing both biomolecule analytes and macrobiomolecules for capillary electrophoresis separation are provided. The reagents comprise a branched polymer. When mixed with a sample containing both biomolecule analytes and macrobiomolecules, particularly DNA templates, the branched polymer of the reagent can suppress the entrance of the macrobiomolecules into a capillary electrophoresis tube during electrokinetic injection of the sample into the capillary electrophoresis tube.
Formulation Of Spotting Solution To Achieve Uniform Spot Size And Morphology And For Nondestructive Quality Control Of Assay Articles
US Patent:
7368082, May 6, 2008
Filed:
Dec 12, 2002
Appl. No.:
10/317950
Inventors:
Daniel Keys - Irvine CA, US
M. Parameswara Reddy - Brea CA, US
M. Parameswara Reddy - Brea CA, US
International Classification:
G01N 15/06, G01N 33/00, G01N 33/48
US Classification:
422 681, 422 8205, 427 9, 427157
Abstract:
This invention relates to spotting solutions for the production of assay articles with uniform spot size and morphology for the detection of biopolymers, comprising N-lauroyl sarcosine in a buffered solution. Optionally the spotting solution may further comprise a fluorophore or a dye. This invention further provides means for non-destructive quality control of the production of said assay articles. This invention further provides means for non-destructive quality control of the production of said assay articles.
Assay System Using Labeled Oligonucleotides
US Patent:
2007024, Oct 18, 2007
Filed:
May 9, 2007
Appl. No.:
11/746520
Inventors:
M. Reddy - Brea CA, US
Daniel Keys - Irvine CA, US
Firdous Farooqui - Brea CA, US
Daniel Keys - Irvine CA, US
Firdous Farooqui - Brea CA, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
C12Q 1/68, G01N 33/543
US Classification:
435006000, 436518000
Abstract:
The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.
Method And Apparatus For Increasing The Dynamic Range And Accuracy Of Binding Assays
US Patent:
6867005, Mar 15, 2005
Filed:
Oct 24, 2001
Appl. No.:
10/032790
Inventors:
Daniel A. Keys - Irvine CA, US
Parameswara M. Reddy - Brea CA, US
Parameswara M. Reddy - Brea CA, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
G01N033/53
US Classification:
435 71, 4352861, 4352872, 4352887, 436164, 436172
Abstract:
This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i. e. , an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.
Cell-Based Microarrays, And Methods For Their Preparation And Use
US Patent:
2006029, Dec 28, 2006
Filed:
Jun 7, 2006
Appl. No.:
11/448003
Inventors:
M. Reddy - Brea CA, US
Kurt Brillhart - Mission Viejo CA, US
Daniel Keys - Irvine CA, US
Kurt Brillhart - Mission Viejo CA, US
Daniel Keys - Irvine CA, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
C12Q 1/70, C12Q 1/68, C12M 1/34
US Classification:
435005000, 435006000, 435287200, 977802000, 977924000
Abstract:
The present invention is in the field of chemistry and biotechnology. The present invention relates to cell-based microarrays, improved methods for forming such arrays, and methods for using such arrays in diagnostics, therapeutics and research. The invention particularly concerns microarrays in which ligands of a target cells are immobilized to the array support via ligand-binding molecules bound to an oligonucleotide that is hybridized to a support-immobilized oligonucleotide.
Method And Apparatus For Increasing The Dynamic Range And Accuracy Of Binding Assays
US Patent:
2005017, Aug 4, 2005
Filed:
Feb 8, 2005
Appl. No.:
11/052219
Inventors:
Daniel Keys - Irvine CA, US
Parameswara Reddy - Brea CA, US
Parameswara Reddy - Brea CA, US
International Classification:
C12Q001/68, G06F019/00, G01N033/48, G01N033/50, C12M001/34
US Classification:
435006000, 702019000, 435287200
Abstract:
This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i.e., an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.
Reducing Microarray Variation With Internal Reference Spots
US Patent:
2004022, Nov 18, 2004
Filed:
May 16, 2003
Appl. No.:
10/439811
Inventors:
M. Reddy - Brea CA, US
Kurt Brillhart - Mission Viejo CA, US
Firdous Farooqui - Brea CA, US
Daniel Keys - Irvine CA, US
Kurt Brillhart - Mission Viejo CA, US
Firdous Farooqui - Brea CA, US
Daniel Keys - Irvine CA, US
International Classification:
C12Q001/68, C12M001/34
US Classification:
435/006000, 435/287200
Abstract:
The present invention provides a system using internal reference spots to reduce microarray assay variation. A microarray device for quantifying a plurality of target ligands in a sample includes a solid support having a plurality of test areas; a plurality of different target capture ligands immobilized onto the test areas; and at least one reference capture ligand immobilized onto the test areas. The reference capture ligand captures a reference ligand not normally present in the sample.
Assay System Using Labeled Oligonucleotides
US Patent:
7229763, Jun 12, 2007
Filed:
Apr 7, 2003
Appl. No.:
10/408626
Inventors:
M. Parameswara Reddy - Brea CA, US
Daniel A. Keys - Irvine CA, US
Firdous Farooqui - Brea CA, US
Daniel A. Keys - Irvine CA, US
Firdous Farooqui - Brea CA, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
C12Q 1/68, G01N 33/53, C12P 19/34, C07H 21/04, A61K 39/395
US Classification:
435 6, 435 71, 435 911, 536 243, 4241301
Abstract:
The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.
Oligonucleotide Pairs For Multiplexed Binding Assays
US Patent:
7195875, Mar 27, 2007
Filed:
Apr 18, 2003
Appl. No.:
10/419020
Inventors:
Daniel A. Keys - Irvine CA, US
Parameswara Meda Reddy - Brea CA, US
Jackie S. Bodnar - Fullerton CA, US
Firdous Farooqui - Brea CA, US
Parameswara Meda Reddy - Brea CA, US
Jackie S. Bodnar - Fullerton CA, US
Firdous Farooqui - Brea CA, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
C12Q 1/68, C07H 21/04
US Classification:
435 6, 536 231
Abstract:
The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.