Inventors:
Eric V. Anslyn - Austin TX, US
Himali Hewage - Austin TX, US
Ronald Houk - Austin TX, US
Damon V. Borich - Austin TX, US
Jason A. Neeser - Austin TX, US
International Classification:
C12Q 1/68, G01N 33/542
Abstract:
Compositions, methods, and systems for detecting small molecules using chemiluminescent signaling assay technology are provided. One system provided herein comprises a chromophore; an oxalate ester, a peroxide, and a modulating agent, wherein the modulating agent will perturb a chemiluminescent signal generated by an interaction among the chromophore, the oxalate ester, and a peroxide; and the perturbation will occur in response to an analyte. One method provided herein comprises combining a chromophore, an oxalate ester, a peroxide, and a modulating agent, wherein: the modulating agent will perturb a chemiluminescent signal generate by an interaction among the chromophore, the oxalate ester, and a peroxide; and the perturbation will occur in response to an analyte. Another method provides a calorimetric or fluorometric signal response in the presence of an analyte.