Inventors:
James Baumgardner - Folsom PA, US
Cynthia Otto - Folsom PA, US
Assignee:
The Trustees of the University of Pennsylvania - Philadelphia PA
International Classification:
C12Q001/00, C12Q001/26, C12M003/00
US Classification:
435004000, 435025000, 435287100
Abstract:
An apparatus and method for measuring nitric oxide production and oxygen consumption in cultures of adherent cells continuously and without destroying the cells. The method involves flowing growth media through a tube having adherent cells are adhered to the inner surface thereof and then contacting the growth media with an NO or Osensor to detect the concentration of NO or Oin the growth media.