CRAIG S MILLER
Dentist at Ashgrove Rd, Nicholasville, KY
License number
Florida 81
Issued Date
Dec 22, 2014
Effective Date
Dec 22, 2014
Expiration Date
Dec 21, 2016
Category
Health Care
Type
Dental Expert Witness Certificate
Address
Address
5026 Ashgrove Rd, Nicholasville, KY 40356
Phone
(859) 433-5755
Professional information
Neuronal Cell Model And Methods Of Use Thereof
US Patent:
6573041, Jun 3, 2003
Filed:
Oct 29, 2001
Appl. No.:
09/984337
Inventors:
Craig S. Miller - Nicholasville KY
Robert J. Jacob - Lexington KY
Robert J. Danaher - Lexington KY
Robert J. Jacob - Lexington KY
Robert J. Danaher - Lexington KY
Assignee:
University of Kentucky Research Foundation - Lexington KY
International Classification:
C12Q 170
US Classification:
435 5, 435 6, 435353, 4352351
Abstract:
We previously described a novel in vitro model of a non-productive herpes simplex virus type 1 (HSV-1) infection in neurally-differentiated (ND)-PC12 cells that allows for inducible virus replication upon forskolin and heat stress (HS) treatment. In this research, we further characterized the model with respect to HSV-2 strain 333. We found that: (i) ND-PC12 cells are non-permissive to HSV-2 replication; (ii) HSV-2 can establish a quiescent infection, like HSV-1, in ND-PC12 cells with the transient use of acycloguanosine (ACV); however unlike HSV-1, anti-viral conditions are not obligatory to establish and maintain a quiescent state; (iii) the quiescent state is maintained in the presence of Vero cell cocultivation indicating that such cultures are free of infectious virus; and (iv) a high percentage of quiescently infected (QIF)-PC 12 cell cultures (80-100%) produce HSV-2 in response to forskolin and HS (43° C. , 3 h) treatment for as long as 4 weeks post infection. These findings indicate that ND-PC12 cells can harbor HSV-2 in a cryptic and non-productive state that is reversible.
Methods And Compositions For Diagnosis Of Acute Myocardial Infarction (Ami)
US Patent:
2012020, Aug 16, 2012
Filed:
Aug 20, 2010
Appl. No.:
13/390619
Inventors:
John T. McDevitt - Houston TX, US
Craig S. Miller - Nicholasville KY, US
Jeffrey L. Ebersole - Lexington KY, US
Nicolaos Christodoulides - Austin TX, US
Pierre N. Floriano - Missouri City TX, US
Craig S. Miller - Nicholasville KY, US
Jeffrey L. Ebersole - Lexington KY, US
Nicolaos Christodoulides - Austin TX, US
Pierre N. Floriano - Missouri City TX, US
International Classification:
C40B 30/04, G01N 33/573, G01N 33/566
US Classification:
506 9, 435 792, 435 74, 436501
Abstract:
Embodiments of the invention utilizes advanced detection methodologies, such as the lab-on-a-chip (LOC) technology, as a cost-effective, efficient, ultra-sensitive rapid method for diagnosing Acute Myocardial Infarction (AMI) in human subjects. In certain aspects, multiple biomarkers of AMI are concurrently detected and measured in serum and saliva to provide a more efficient, sensitive and accurate diagnosis of AMI.
Neuronal Cell Model And Methods Of Use Therefor
US Patent:
6268124, Jul 31, 2001
Filed:
Mar 19, 1999
Appl. No.:
9/272158
Inventors:
Craig S. Miller - Nicholasville KY
Robert J. Danaher - Lexington KY
Robert J. Jacob - Lexington KY
Robert J. Danaher - Lexington KY
Robert J. Jacob - Lexington KY
Assignee:
University of Kentucky Research Foundation - Lexington KY
International Classification:
C12Q 170, C12N 506
US Classification:
435 5
Abstract:
The present invention provides, inter alia, a PC12 cell quiescently infected with a neurotropic virus. Preferred quiescently-infected neural cells are those wherein the neurotropic virus is a neurotropic herpes virus. Methods related to these and other materials are also provided.