CHRISTOPHER WALSH
Broker in Newton, MA
License number
Massachusetts 9522334
Issued Date
Dec 29, 2011
Expiration Date
Jul 22, 2018
Type
Salesperson
Address
Address
Newton, MA 02466
Professional information
Production, Purification, Cleavage And Use Of Fusion Peptides
US Patent:
5648244, Jul 15, 1997
Filed:
Sep 27, 1993
Appl. No.:
8/127692
Inventors:
Athan Kuliopulos - Cambridge MA
Christopher T. Walsh - West Newton MA
Christopher T. Walsh - West Newton MA
Assignee:
President and Fellows of Harvard College - Cambridge MA
International Classification:
C12N 1509, C12N 1563, C12N 1570, C12N 1562
US Classification:
435 697
Abstract:
A method for producing a fusion peptide. A vector is provided with nucleic acid encoding a carrier peptide and at the 3' end of the nucleic acid, a unidirectional restriction endonuclease cleavage site recognized by a restriction endonuclease with the ability to create a non-palindromic 3-base overhang. The vector is cleaved with the restriction endonuclease to produce a cleaved vector. One or more nucleic acids encoding a desired peptide and having at least a 3-base overhang at each end configured and arranged for ligation with the cleaved vector is then ligated to the cleavage site.