CHRISTOPHER R MILLER
Plumbers in Arlington, MA
License number
Massachusetts 21246
Issued Date
May 20, 1988
Expiration Date
May 1, 2004
Type
Journeyman Plumber
Address
Address
Arlington, MA 02474
Professional information
Oligonucleotide Arrays To Monitor Gene Expression And Methods For Making And Using Same
US Patent:
2006001, Jan 12, 2006
Filed:
May 11, 2005
Appl. No.:
11/128049
Inventors:
Mark Melville - Melrose MA, US
Timothy Charlebois - Andover MA, US
William Mounts - Andover MA, US
Louane Hann - Boston MA, US
Martin Sinacore - Andover MA, US
Mark Leonard - Manchester NH, US
Eugene Brown - Newton Highlands MA, US
Christopher Miller - Arlington MA, US
Gene Lee - Chelmsford MA, US
Timothy Charlebois - Andover MA, US
William Mounts - Andover MA, US
Louane Hann - Boston MA, US
Martin Sinacore - Andover MA, US
Mark Leonard - Manchester NH, US
Eugene Brown - Newton Highlands MA, US
Christopher Miller - Arlington MA, US
Gene Lee - Chelmsford MA, US
International Classification:
A01H 1/00, C12N 15/82
US Classification:
800278000
Abstract:
The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
Novel Polynucleotides Related To Oligonucleotide Arrays To Monitor Gene Expression
US Patent:
2006000, Jan 5, 2006
Filed:
May 11, 2005
Appl. No.:
11/128061
Inventors:
Mark Melville - Melrose MA, US
Timothy Charlebois - Andover MA, US
William Mounts - Andover MA, US
Louane Hann - Boston MA, US
Martin Sinacore - Andover MA, US
Mark Leonard - Manchester MA, US
Eugene Brown - Newton Highlands MA, US
Christopher Miller - Arlington MA, US
Gene Lee - Chelmsford MA, US
Timothy Charlebois - Andover MA, US
William Mounts - Andover MA, US
Louane Hann - Boston MA, US
Martin Sinacore - Andover MA, US
Mark Leonard - Manchester MA, US
Eugene Brown - Newton Highlands MA, US
Christopher Miller - Arlington MA, US
Gene Lee - Chelmsford MA, US
International Classification:
A61K 48/00, C07K 14/47, C12Q 1/68, C07H 21/04, C12N 5/06
US Classification:
514044000, 435006000, 435069100, 435358000, 530350000, 536023500
Abstract:
The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
Nucleic Acid Encoding A Transcription Factor, Idx-1
US Patent:
6210960, Apr 3, 2001
Filed:
Nov 19, 1996
Appl. No.:
8/751344
Inventors:
Joel F. Habener - Newton Highlands MA
Christopher P. Miller - Arlington MA
Christopher P. Miller - Arlington MA
Assignee:
The General Hospital Corporation - Boston MA
International Classification:
C12N 1263, C12N 1512, C12N 510
US Classification:
4353201
Abstract:
The invention features a novel recombinant polypeptide that transactivates the somatostatin promoter, the polypeptide being present in pancreatic duct cells and not present in pancreatic. alpha. -cells, the polypeptide being encoded by a gene which encodes a protein on the order of 31 kd.
Transcription Factor And Uses Therefor
US Patent:
5858973, Jan 12, 1999
Filed:
Feb 23, 1994
Appl. No.:
8/202044
Inventors:
Joel F. Habener - Newton Highlands MA
Christopher P. Miller - Arlington MA
Christopher P. Miller - Arlington MA
Assignee:
The General Hospital Corporation - Boston MA
International Classification:
A61K 3800, A61K 3816, A61K 3831
US Classification:
514 12
Abstract:
The invention features a novel recombinant polypeptide that transactivates the somatostatin promoter, the polypeptide being present in pancreatic duct cells and not present in pancreatic. alpha. -cells, the polypeptide being encoded by a gene which encodes a protein on the order of 31 kd.