CHRISTOPH SEEGER
Pilots at Waring Rd, Melrose Park, PA
License number
Pennsylvania A5220310
Issued Date
Dec 2016
Expiration Date
Dec 2018
Category
Airmen
Type
Authorized Aircraft Instructor
Address
Address
407 Waring Rd, Melrose Park, PA 19027
Professional information
Hepadnavirus Polymerase Gene Product Having Rna-Dependent Dna Priming And Reverse Transcriptase Activities And Methods Of Measuring The Activities Thereof
US Patent:
5334525, Aug 2, 1994
Filed:
Aug 28, 1992
Appl. No.:
7/937214
Inventors:
Christoph Seeger - Melrose Park PA
Assignee:
Fox Chase Cancer Center - Philadelphia PA
International Classification:
C12N 912, C12N 1510, C08L 500, C12Q 148
US Classification:
435194
Abstract:
An enzymatically active, substantially virus-free hepadnavirus polymerase gene product is provided, which possesses a DNA priming function and reverse transcriptase activity, both of which are dependent on the presence of an RNA template and magnesium ions. The hepadnavirus polymerase gene product is produced by in vitro transcription and translation of a hepadnavirus pol gene.
The enzymatically active hepadnavirus polymerase gene product is used in rapid in vitro assays to screen potential anti-hepadnaviral agents. An assay for the DNA priming activity of the gene product is provided, wherein covalent attachment of labelled dGTP to the gene product is quantitatively measured. An assay for the reverse transcriptase activity of the gene product is provided, wherein incorporation of nucleotide triphosphates into the nascent DNA minus strand is measured, taking advantage of the covalent attachment of said nascent strand to the polymerase gene product.
Cultured Cell Line That Inducibly Expresses The Hepatitis B Virus Genome, And Uses Thereof For Screening Antiviral Substances
US Patent:
5723319, Mar 3, 1998
Filed:
Jun 5, 1995
Appl. No.:
8/462216
Inventors:
Robert W. King - West Chester PA
Christopher S. Barker - Huntington Valley PA
Christoph Seeger - Melrose Park PA
Christopher S. Barker - Huntington Valley PA
Christoph Seeger - Melrose Park PA
Assignee:
Avid Therapeutics, Inc. - Philadelphia PA
Fox Chase Cancer Center - Philadelphia PA
Fox Chase Cancer Center - Philadelphia PA
International Classification:
C12N 1551, C12N 510, C12Q 170
US Classification:
435 693
Abstract:
Novel cell lines and methods are provided for inducibly expressing the Hepatitis B virus (HBV) genome within cultured cells. Cells are stably transformed with an HBV genome under control of an operator/promoter target sequence which can activate expression of the HBV genome when bound by a cognate transactivator protein. The cell lines are also stably transformed with a gene that encodes and expresses a suitable transactivator protein. Cells may be maintained under non-inducing conditions whereby the transactivator protein is unable to interact with the operator/promoter target sequence, so the HBV genome is not expressed. Under appropriate inducing conditions, the transactivator protein is enabled to interact with the operator/promoter target sequence, thereby activating expression of the HBV genome. The inducible system provides an effective and rapid cell-based assay to screen compounds for anti-hepadnaviral activity.
Hepadnavirus Polymerase Gene Product Having Rna-Dependent Dna Priming And Reverse Transcriptase Activities And Methods Of Measuring The Activities Thereof
US Patent:
5360714, Nov 1, 1994
Filed:
Aug 2, 1993
Appl. No.:
8/101253
Inventors:
Christoph Seeger - Melrose PA
Assignee:
Fox Chase Cancer Center - Philadelphia PA
International Classification:
C12Q 148, C12Q 168, C08L 500, C12N 912
US Classification:
435 5
Abstract:
An enzymatically active composition of matter, comprising an extracellular combination of a substantially virus-free hepadnavirus polymerase gene product and an RNA template for hepadnavirus minus strand DNA synthesis, is provided. The composition possesses a DNA priming activity and a reverse transcriptase activity. The hepadnavirus polymerase gene product is produced by in vitro expression of a hepadnavirus pol gene.
The enzymatically active composition of matter is used in rapid in vitro assays to screen potential anti-hepadnaviral agents. Assays for various functions of the hepadnavirus DNA polymerase are provided, including: (1) binding of the polymerase gene product to hepadnavirus pre-genomic RNA; (2) DNA priming activity of the polymerase gene product; and (3) reverse transcriptase activity of the polymerase gene product.
Replication Of Hepatitis C Virus In Non-Hepatic Epithelial And Mouse Hepatic Cells
US Patent:
7416840, Aug 26, 2008
Filed:
Dec 12, 2003
Appl. No.:
10/536955
Inventors:
Qing Zhu - Emeryville CA, US
Christoph Seeger - Elkins Park PA, US
Christoph Seeger - Elkins Park PA, US
Assignee:
Fox Chase Cancer Center - Philadelphia PA
International Classification:
C12Q 1/70, C12N 5/10, C12N 15/87
US Classification:
435 5, 435 32, 435354, 435366, 435367, 435455, 4242281, 424 92
Abstract:
Cells and cell lines which replicate HCV of non-hepatic human and non human origin are disclosed. Also provided are methods of using such cells and cell lines to identify anti-HCV agents for the treatment of HCV infection.