CHARLES S RICHARDSON
Broker in Boston, MA

5639608, Jun 17, 1997

Feb 14, 1994

8/196538

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President & Fellows of Harvard College - Cambridge MA

C12Q 168, C12P 1934

435 6

This invention relates to methods for determining the nucleotide base sequence of a deoxyribose nucleic acid molecule comprising the steps of incubating the nucleic acid molecule with an oligonucleotide primer of 5 to 8 bases in length, a plurality of deoxynucleoside triphosphates, at least one chain terminating agent, and a T7-type DNA polymerase having less than 500 units of exonuclease activity under conditions in which the primer is extended until the chain terminating agent is incorporated, and separating the products of the incubating step according to size, whereby at least a part of the nucleotide base sequence of the nucleic acid molecule can be determined.

5534407, Jul 9, 1996

Apr 18, 1994

8/229396

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12Q 170, C12Q 168

435 5

Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i. e. , one in which the t. sub. 1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i. e. , to a t. sub. 1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min. ) in the absence of nucleotide triphosphates.

5614365, Mar 25, 1997

Nov 10, 1994

8/337615

Stanley Tabor - Cambridge MA
Charles Richardson - Chestnut Hill MA

President & Fellow of Harvard College - Cambridge MA

C12Q 168, C12P 1934, C12N 900, C07H 2102

435 6

Modified gene encoding a modified DNA polymerase wherein the modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase.

5122345, Jun 16, 1992

Jul 3, 1990

7/547870

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

G01N 0000

422116

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U. S. Public Health Service, contract number AI-06045. The U. S. government has certain rights in the invention.

5266466, Nov 30, 1993

Sep 4, 1992

7/940531

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12P 1934, C12N 912, C12Q 168

435 915

This invention relates to T7-type DNA polymerases and methods for using them.

5972603, Oct 26, 1999

May 31, 1996

8/656555

Ella Bedford - Brookline MA
Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12Q 168, C12Q 170, C07K 100

435 6

Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

4962020, Oct 9, 1990

Jul 12, 1988

7/218103

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12Q 168

435 6

A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

5409811, Apr 25, 1995

Apr 16, 1992

7/869520

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12Q 168, C12P 1934

435 6

A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.

4946786, Aug 7, 1990

Mar 1, 1990

7/487746

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12N 912, C12N 1500, C12N 120, C12N 700

435194

1Method for production of a composition consisting essentially of a T7-type DNA polymerase and thioredoxin. The method includes culturing a cell containing plasmid DNA encoding a T7-type DNA polymerase to express the T7-type DNA polymerase from the plasmid DNA, and purifying the T7-type DNA polymerase expressed from the cell to reduce the exonuclease activity associated with the T7-type DNA polymerase compared to the level of exonuclease activity associated with a corresponding naturally-occurring T7-type DNA polymerase.

4921794, May 1, 1990

Dec 5, 1988

7/279990

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

President and Fellows of Harvard College - Cambridge MA

C12P 1934, C12N 1500, C12N 912, C07H 1512

435 91

This invention relates to T7-type DNA polymerases and methods for amplification of DNA, for example by polymerase chain reaction.