CARLOS J. RODRIGUEZ, DPM, MS, PA
Medical Practice at 27 Ave, Miami, FL

5616501, Apr 1, 1997

Nov 17, 1995

8/560423

Carlos M. Rodriguez - Miami FL
Stephen L. Ledis - Hialeah FL

Coulter Corporation - Miami FL

G01N 3348

436 63

A reticulocyte analyzing method and apparatus in which a biological sample stream of ghosted red cells is passed into and through a point focused beam of light, such as laser light. A light detector structure is positioned with respect to the axis of the light beam to provide a light output pulse indicative of the passage of each cell. Electrically conductive contacts within the fluid stream can provide additional electrical pulse outputs of each cell. A staining reagent can be utilized with a ghosting reagent to further differentiate the reticulocytes. The light and the light and electronic produced output pulses or signals can be combined to define and quantify reticulocytes as distinct from other known cell types.

6159740, Dec 12, 2000

May 17, 1990

7/525231

James Carey Hudson - Miami FL
Thomas Russell - Miami FL
Carlos M. Rodriguez - Miami FL
Wallace H. Coulter - Miami Springs FL

Coulter Corporation - Miami FL

G01N 3350, G01N 3353, G01N 3380

436 63

A method and apparatus for performing screening of obscured or partially obscured cells to enumerate one or more cell population subsets. For example, a whole blood sample or portion thereof is screened to provide the desired analysis of one or more white blood cell population subsets in the sample. A white blood cell population including the obscured subset of interest is first counted, along with a standard population. The standard population can be one of the total number of white blood cell populations, a second white blood cell population which does not obscure the shifted or non-shifted sensed characteristic of the subset of interest, an artificial population formed by microspheres which also do not obscure the shifted or non-shifted sensed characteristic of the subset of interest or a white blood cell population into which the sensed characteristic of the subset will be wholly or partially shifted. The sensed characteristic of the white blood cell population subset of interest then is shifted by binding microspheres having monoclonal antibodies specific to the white blood cell population subset of interest to the cell population. The white blood cell population, and the standard population then are again counted and compared to the original counts to obtain an enumeration of the white blood cell population subset of interest.

4791355, Dec 13, 1988

Oct 21, 1986

6/921654

Wallace H. Coulter - Miami Springs FL
Carlos M. Rodriguez - Miami FL

Coulter Electronics Inc. - Hialeah FL

G01N 2700

324 711

A circuit, which is useful to provide data needed to measure the electrical opacity of a particle, for instance a blood cell, passing through a Coulter type transducer, includes a current source for providing a conventional d. c. current through the sensing aperture of the transducer, as well as an oscillator for providing a high frequency current through the aperture. The oscillator includes an active device and a resonant circuit, and the aperture is coupled in parallel with the resonant circuit of the oscillator. The oscillator can be any type of oscillator, such as a Hartley oscillator. As a particle passes through the aperture, the resistance of the aperture increases, which in turn, increases the Q of the oscillator circuit, whereby the oscillator output signal is amplitude modulated in accordance with the increased Q. This amplitude modulated signal can be detected to provide a value based on the high frequency reactance of the particle being detected. The change in d. c.