CARL T. WITTWER, MD
Medical Practice at Medical Dr, Salt Lake City, UT

6503720, Jan 7, 2003

Feb 20, 2001

09/789170

Carl T. Wittwer - Salt Lake City UT
Martin Gutekunst - Eberfind, DE
Sabine Lohmann - Iffeldorf, DE

Roche Diagnostics GmbH
University of Utah Research Foundation - Salt Lake City UT

C12Q 168

435 6, 435 912, 435 39, 536 243, 536 2532

Methods are provided for quantifying the concentration of a nucleic acid in a nucleic acid sample. The methods include contacting the nucleic acid sample with an amplifying agent, amplifying at least one predetermined locus of the nucleic acid by subjecting the sample to a number of amplification, generating an amplification curve or array, calculating the first, second or n th order derivative of the amplification curve or array, determining a maximum, minimum, or zero value of the derivative, and using the maximum, minimum, or zero value to calculate the initial concentration of the nucleic acid in the nucleic acid sample.

6472156, Oct 29, 2002

Aug 30, 2000

09/651374

Carl T. Wittwer - Salt Lake City UT
Mark G. Herrmann - Salt Lake City UT

The University of Utah - Salt Lake City UT

C12Q 168

435 6, 435 912, 436 94, 536 2532

The invention provides methods and devices for analyzing sequence variations in nucleic acid samples comprising multiple loci, each having two, three or more possible allelic sequences. The method involves combining at least a first and second pair of oligonucleotide probes with the nucleic acid sample. The first pair of probes is capable of hybridizing in proximity to each other within a segment of the nucleic acid sample comprising the first locus and the second pair is capable of hybridizing in proximity to each other within a segment of the nucleic acid sample comprising the second locus. The first member of each probe pair comprises a FRET donor and the second member comprises a FRET acceptor, the FRET acceptor of the first probe pair member having a different emission spectrum from the FRET acceptor of the second probe pair. Upon hybridization, the proximity of the first and second member of each probe pair is sufficient to allow fluorescence resonance energy transfer between the FRET donor and the FRET acceptor.

2002015, Oct 17, 2002

Aug 12, 1999

09/373041

CARL T. WITTWER - SALT LAKE CITY UT, US
MARK G. HERRMANN - CLINTON UT, US
DAVID M. HEAP - BOUNTIFUL UT, US

C12M001/40

435/286100, 435/287200, 435/303100, 435/091200, 392/323000, 204/601000

A system for thermal cycling a sample utilizing electrolyte conductance heating. An electric current is passed through the sample to increase its temperature due to resistance heating. As the sample acquires more thermal energy its viscosity changes, which causes a change in resistance. Because of this characteristic, temperature of the sample can be measured as a function of resistance and temperature can be controlled using resistance of the solution as feedback to a circuit which controls the heating of the sample by electrical conductance.

6140054, Oct 31, 2000

Sep 30, 1998

9/164023

Carl T. Wittwer - Salt Lake City UT
Philip S. Bernard - Salt Lake City UT

University of Utah Research Foundation - Salt Lake City UT

C12Q 168

435 6

The present invention is directed to a mutation detection kit and method of analyzing multiple loci of one or more nucleic acid sequences for the presence of mutations or polymorphisms. More particularly, the present invention relates to the use of the polymerase chain reaction (PCR) and fluorescently labeled oligonucleotide hybridization probes to identify mutations and polymorphisms based on melting curve analysis of the hybridization probes.

2012030, Nov 29, 2012

Nov 17, 2011

13/299040

Carl T. Wittwer - Salt Lake City UT, US
Gudrun Reed - Salt Lake City UT, US
Virginie Dujols - Sandy UT, US
Luming Zhou - Salt Lake City UT, US

G01N 21/64

435 611, 435 612, 422 8208

Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

7238321, Jul 3, 2007

Jul 13, 2004

10/891161

Carl T. Wittwer - Salt Lake City UT, US
David R. Hillyard - Salt Lake City UT, US

University of Utah Research Foundation - Salt Lake City UT

G01N 1/00, G01N 15/00, B10J 19/18, C12P 19/34

422 50, 422 681, 422134, 435 912

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achieved by the present invention.

7803551, Sep 28, 2010

Aug 11, 2009

12/500860

Carl T. Wittwer - Salt Lake City UT, US
Virginie Dujols - Sandy UT, US

University of Utah Research Foundation - Salt Lake City UT
Idaho Technology, Inc. - Salt Lake City UT

C12Q 1/68, C12P 19/34, C07H 21/04

435 6, 435 912, 536 2431, 536 24

Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

2003016, Sep 4, 2003

Feb 12, 2002

10/074178

David Eyre - Salt Lake City UT, US
Carl Wittwer - Salt Lake City UT, US

C12Q001/68, G06F019/00, G01N033/48, G01N033/50, C12P019/34

435/006000, 435/091200, 702/020000

A method and device are described for analyzing a sample for the presence of a nucleic acid wherein the sample is amplified, illustratively using PCR, in the presence of a fluorescent probe capable of providing a signal related to the quantity of nucleic acid present. A nucleic acid sample is amplified in the presence of the fluorescent entity, and the fluorescence intensity is measured at each of a plurality of amplification cycles. Scores are obtained from various tests performed on the fluorescence data, and the scores are used to determine whether the nucleic acid is present in the sample.

2013006, Mar 14, 2013

Aug 8, 2012

13/569701

Bruce K. Gale - Taylorsville UT, US
Niel Davenport Crews - Ruston LA, US
Carl Thomas Wittwer - Salt Lake City UT, US

C12P 19/34, B32B 38/04, B32B 38/14, G01N 21/64, B32B 37/14

435 612, 435 912, 156277, 156256

Disclosed are compositions and a method for amplification and detection of nucleic acid sequences based on continuous flow thermal gradient PCR.