C. RONALD SCOTT
Medical Practice at Sand Point Way, Seattle, WA

6670194, Dec 30, 2003

Aug 25, 1999

09/383062

Rudolf Hans Aebersold - Mercer Island WA
Michael H. Gelb - Seattle WA
Steven P. Gygi - Seattle WA
C. Ronald Scott - Seattle WA
Frantisek Turecek - Seattle WA
Scott A. Gerber - Seattle WA
Beate Rist - Seattle WA

University of Washington - Seattle WA

C07C33116

436173, 436 86, 436 89, 436120, 436161, 436165, 436166, 436167, 436168, 436171, 436174, 436175, 436177, 530350, 530812, 5303915

Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e. g. , by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e. g. , products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples.

7544518, Jun 9, 2009

Nov 23, 2004

10/994815

Rudolf Hans Aebersold - Mercer Island WA, US
Michael H. Gelb - Seattle WA, US
Steven P. Gygi - Seattle WA, US
C. Ronald Scott - Seattle WA, US
Frantisek Turecek - Seattle WA, US
Scott A. Gerber - Seattle WA, US
Beate Rist - Seattle WA, US

University of Washington - Seattle WA

G01N 33/68

436173, 436 86, 436 89, 436120, 436161, 436165, 436166, 436167, 436168, 436171, 436174, 436175, 436177, 530350, 530812, 5303915

Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e. g. , by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e. g. , products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples.

6852544, Feb 8, 2005

Apr 20, 2001

09/839884

Rudolf Hans Aebersold - Mercer Island WA, US
Michael H. Gelb - Seattle WA, US
Steven P. Gygi - Seattle WA, US
C. Ronald Scott - Seattle WA, US
Frantisek Turecek - Seattle WA, US
Scott A. Gerber - Seattle WA, US
Beate Rist - Seattle WA, US

University of Washington - Seattle WA

G01N033/68

436173, 436 86, 436 89, 436120, 436161, 436165, 436166, 436167, 436168, 436171, 436174, 436175, 436177, 530350, 530812, 5303915

Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e. g. , by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e. g. , products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples.