Brian William Fouty
Physician at Northbrook Cir, Spanish Fort, AL
License number
Colorado 31770
Issued Date
Jun 19, 1992
Renew Date
May 1, 2015
Expiration Date
Apr 30, 2017
Type
Physician
Address
Address
7277 Northbrook Cir, Spanish Fort, AL 36527
Professional information
Method For Increasing Retroviral Infectivity
US Patent:
2012002, Feb 2, 2012
Filed:
Jun 28, 2011
Appl. No.:
13/170836
Inventors:
Victor Solodushko - Mobile AL, US
Brian Fouty - Spanish Fort AL, US
Brian Fouty - Spanish Fort AL, US
Assignee:
UNIVERSITY OF SOUTH ALABAMA - Mobile AL
International Classification:
C12N 15/867, C12N 5/071
US Classification:
435456, 435375
Abstract:
The present invention is directed to methods for enhanced retroviral delivery of a nucleic acid to a target cell in vitro, ex vivo or in vivo, which involves increasing infectivity of a retrovirus carrying a transgene of interest to a target cell. In one particular aspect the invention relates to a method for increasing retroviral infectivity of target cells comprising culturing packaging cells transfected with retroviral vector containing a transgene of interest in medium containing a glucocorticoid receptor agonist or analog or derivative thereof present in the medium in an amount effective to increase titer of propagated retrovirus; and subsequently culturing a target cell in medium comprising the propagated retrovirus from a) and a glucocorticoid receptor antagonist or analog or derivative thereof present in the medium in an amount effective to increase target cell sensitivity to infection by the propagated retrovirus.