DR. BORIS M. PETERLIN, MD
Osteopathic Medicine at Parnassus Ave, San Francisco, CA

6025124, Feb 15, 2000

Nov 19, 1996

8/752722

Melanie Adams - San Francisco CA
Joseph Romeo - Palo Alto CA
Boris Matija Peterlin - San Francisco CA
Michael Paul Busch - Madera CA

The Regents of the University of California - Oakland CA

C12Q 168, C07H 2104, C12P 1934

435 5

A TAR marker, method for detection and monitoring of HIV latency and activation and an assay for detection of the marker. The assay sensitively detects HIV transcription and monitors HIV transcriptional activity by detecting the presence of TAR fragments and full length transcripts, quantifying both and determining the ratio of short to long transcripts. A low ratio correlates with a latent-type transcriptional activity of HIV whereas the appearance of long transcripts signifies increased efficiency of transcriptional activity of HIV and the transition from latency to activation. The size difference between the TAR fragments appearing predominantly in latency and the full length transcripts appearing predominantly during the HIV activation is detected by RT-PCR assay that utilizes novel primers and probes. The obtained ratio is a sensitive tool in detection of HIV infection, the analysis of load of latent and active virus and monitoring the transition from the latent to active state of HIV replication.

5576176, Nov 19, 1996

Mar 3, 1994

8/206384

Melanie Adams - San Francisco CA
Joseph Romeo - San Francisco CA
Boris M. Peterlin - San Francisco CA
Michael P. Busch - Corte Madera CA

The Regents of the University of California - Oakland CA

C07H 2104, C12Q 170, C12Q 168, C12P 1934

435 5

A marker and method for detection and monitoring of HIV latency and activation and an assay for detection of the marker. The assay sensitively detects HIV transcription and monitors HIV transcriptional activity by detecting the presence of short and long transcripts, quantifying both and determining the ratio of short to long transcripts. Short transcripts are abundant and a low ratio correlates with a latent-type transcriptional activity of HIV whereas the appearance of long transcripts signifies increased efficiency of transcriptional activity of HIV and the transition from latency to activation. The size difference between the TAR fragments appearing predominantly in latency and the full length transcripts appearing predominantly during the HIV activation is detected by RT-PCR assay that utilizes novel primers and probes. The results are expressed as a ratio of short to long transcripts. The obtained ratio is a sensitive tool in detection of HIV infection, the analysis of load of latent and active virus and monitoring the transition from the latent to active state of HIV replication.