Inventors:
Bobak R. Mozayeni - Rockville MD
Assignee:
The United States of America as represented by the Department of Health
and Human Services - Washington DC
International Classification:
B01D 6308
Abstract:
An apparatus and process for continuous, cell-free, in vitro synthesis of peptides, particularly peptide-MHC complexes make use of a novel bioreactor flow cell which allows for the reproducible, systematic variation of single parameters in order to optimize translation processes. The bioreactor flow cell includes a pair of substantially parallel membranes positioned within a chamber to define therebetween a space, the ratio of total membrane surface area to chamber volume being at least 5 units. sup. -1, which permits high perfusion rates through the system with lower flux rate per membrane area. The apparatus also may include a novel countercurrent flow dialysis cell and other components.