Inventors:
Baruch S. Blumberg - Philadelphia PA
Irving Millman - Willow Grove PA
Assignee:
Institute for Cancer Research - Philadelphia PA
International Classification:
G01N 3316, A61K 4300, G21H 502
Abstract:
A test reagent reagent for HB. sub. s A. sub. g is prepared by applying to a suitable substrate, such as a polystyrene bead, a first coat of purified HB. sub. s A. sub. g to an extent which will permit complete coverage by anti-HB. sub. s. After washing HB. sub. s A. sub. g coated substrate, serum containing anti-HB. sub. s, or an IgG fraction of serum containing anti-HB. sub. s, is applied as a second coat as a second coat in an amount which substantially completely occupies the reacting sites of the HB. sub. s A. sub. g on the outside of the substrate and leaves an excess of antibody reacting groups in a free state, and any unattached material is washed off.
A test sample which is to be tested for HB. sub. s A. sub. g is then contacted with the reagent thus produced, permitting attachment of HB. sub. s A. sub. g if present, and washing is done.