BARBARA V MURZA
Medical Practice at 148 Ct, Miami, FL
License number
Florida 26675
Issued Date
Jan 6, 1988
Effective Date
Oct 11, 2004
Expiration Date
Jun 30, 2002
Category
Health Care
Type
Clinical Laboratory Personnel
Address
Address
10819 SW 148Th Ct, Miami, FL 33196
Professional information
Cyanide-Free Lytic Reagent Composition And Method Of Use For Hemoglobin And White Blood Cell Measurement
US Patent:
7235404, Jun 26, 2007
Filed:
May 4, 2005
Appl. No.:
11/125395
Inventors:
Russell F. Lang - Pembroke Pines FL, US
Dennisse Parra-Diaz - Miami FL, US
Luisa Oramas - Weston FL, US
Barbara Murza - Miami FL, US
Susana Maldonado - Miami FL, US
Dennisse Parra-Diaz - Miami FL, US
Luisa Oramas - Weston FL, US
Barbara Murza - Miami FL, US
Susana Maldonado - Miami FL, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
G01N 33/72, G01N 31/00
US Classification:
436 66, 436 8, 436 10, 436 17, 436 63, 436164, 436166, 436174, 435 2, 2524081
Abstract:
A cyanide-free lytic reagent composition and method of use for measuring the total hemoglobin concentration and white blood cells in a blood sample are disclosed. The lytic reagent composition includes a quaternary ammonium surfactant to lyse red blood cells and release hemoglobin and a ligand to form a stable chromogen with the hemoglobin. The lytic reagent composition has a pH in a range of 3 to 10. The lytic reagent composition can also include a second quaternary ammonium surfactant. The ligand can be malic acid, malonic acid, ethylene diamine, N,N-diethylethylene diamine, N,N′-diethylethylene diamine, diethylene triamine, tetraethylene pentamine, 1,6-hexanediamine, 1,3-pentanediamine, 2-methylpentamethylenediamine, 1,2-diaminocyclohexane, 4-aminoacetophenone, bis-hexamethylenetriamine, pyridazine, or 3,6-dihyroxypyridazine.
Low Formaldehyde Producing Blood Diluent
US Patent:
2003016, Sep 4, 2003
Filed:
Jan 24, 2002
Appl. No.:
10/056617
Inventors:
Russell Lang - Pembroke Pines FL, US
Iris Payan - Pembroke Pines FL, US
Barbara Murza - Miami FL, US
Luisa Oramas - Weston FL, US
Iris Payan - Pembroke Pines FL, US
Barbara Murza - Miami FL, US
Luisa Oramas - Weston FL, US
International Classification:
G01N031/00
US Classification:
436/018000, 436/008000, 436/010000, 436/063000, 436/130000, 252/408100
Abstract:
A low formaldehyde containing aqueous blood diluent contains an effective amount of ethylenediamine tetraacetic acid, ethylenediamine tetraacetic acid derivative, or combinations thereof; an effective amount of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one; and an effective amount of 5-bromo-5-nitro-1,3-dioxane. Advantageously, less than about 1 parts per million of formaldehyde is produced in this multipurpose diluent. A method of analyzing a blood sample containing blood cells is conducted by forming a diluted blood sample by mixing a blood sample containing blood cells with this diluent and analyzing the diluted blood sample to determine a physical parameter of the blood cells.