Work:
College Of Pharmacy And Health Sciences, St. John's University
Lab Manager/ Supervisor
St. John's University
Teaching Fellow
St. John's University - Queens, NY
Teaching Assistant
Education:
St. John's University - Queens, NY
PhD in Pharmacology
St. John's University - Queens, NY
Master of Science in Pharmacology
Dr. Bhanuben Nanavati College of Pharmacy - Mumbai, Maharashtra
Bachelor of Pharmacy
Skills:
Cellular Biology: Cell Culture, microplate-assay development, including maintaining cell lines and design and perform Cellular-based transport assay including Rapid Filtration Methods, Monolayer transport, Inside-out membrane vesicle preparations by Nitrogen Cavitation Methods. Cell viability assays i.e. Colony formation, Drug accumulation and efflux assays, MTT/MTS/XTT/SRB assays. Molecular Biology: Cell-line Stabilization through Stable Transfection and Electroporation Techniques, Cellular Fractionation including Protein, Mitochondria, DNA and RNA Isolation and Detection, biomarker identification, Enzyme Activity and Kinetic Assays, Flowcytometry sample preparation and data analysis (FACS). Biochemistry: Western Blots, ELISA, Immunoprecipitation and Immunohistocytochemistry. In Vivo and Ex Vivo: Whole Animal Pharmacology, including drug administration (i.p, SC, Tail Vein), blood collection (retro- orbital, tail tippling, cardiac puncture). Developing tumor xenograft mouse models. Ex Vivo Assays isolating various organs, setting up the specific organ baths and analyzing acquired data via BioPac systems. Microscopy: Confocal, light, scanning electron and fluorescence microscopy. Chromatography/ Pharmacokinetics: Developing techniques for novel agents using HPLC and analyzing blood samples to determine plasma concentration for analysis of DMPK parameters. -Computers: PC and Mac Operation, Basic Web Designing (HTML), Microsoft Office Applications. Some Database and Analytical Softwares e.g., Prism, SPSS, Sigma Plot, Image J etc. Flowjo for analysis of FACS data. HPLC data analysis (Empowers-Waters and ChemStation-Agilent). Adobe Photoshop CS6, Lightroom 4, Final cut Pro. Skilled digital photography and studio photography.